Somatic Embryogenesis of Finger Millet (Eleusine coracana (L.) Gaertn) using Seed explants
| dc.contributor.advisor | Feyissa, Tileye (PhD) | |
| dc.contributor.author | Gebremedhen, Helen | |
| dc.date.accessioned | 2018-06-18T06:19:49Z | |
| dc.date.accessioned | 2023-11-18T09:50:18Z | |
| dc.date.available | 2018-06-18T06:19:49Z | |
| dc.date.available | 2023-11-18T09:50:18Z | |
| dc.date.issued | 2014-01 | |
| dc.description.abstract | Finger millet (Eleusine coracana subsp. coracana) is one of the most important crops in Africa and India. Finger millet contains different nutrients and is a popular food among diabetic patients in different countries. Finger millet is an orphan crop of developing world on which little research has been done. The improvement of this crop is crucial towards abiotic and biotic stress tolerance. Tissue culture and transformation techniques are among the approaches to improve this crop. Therefore, the objective of this study is to develop an efficient somatic embryogenesis protocol for regeneration of different finger millet cultivars. Somatic embryogenesis protocol for regeneration of four cultivars of finger millet is developed. For all cultivars, mature seeds were used for callus induction. After sterilization of the seeds, callus was induced efficiently in different concentrations of 2, 4-D in all cultivars. From 95 to100% callus induction were recorded on callus induction medium containing 1.0, 1.5, 2.0, 2.5 and 3.0 mg/l 2, 4-D. MS-medium supplemented with 1mg/l 2, 4-D resulted in 45 to 58.3% somatic embryogenesis and 1mg/l KN in combination with 0.25mg/l NAA was resulted with highest mean number (15.85±0.63) of shoots and 16.74±1.86 roots were recorded from AAUFM-19. Key Words: callus induction, Kinetin, NAA, shoot regeneration, somatic embryogenesis | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/1114 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | callus induction;, Kinetin; NAA,; shoot regeneration;somatic embryogenesis | en_US |
| dc.title | Somatic Embryogenesis of Finger Millet (Eleusine coracana (L.) Gaertn) using Seed explants | en_US |
| dc.type | Thesis | en_US |