Inivitro Investigation of Antimicrobial Activities of Albizia Gummifera And Albizia Anthelmintica on Major Bacterial Urop Athogens Isola Ted from Adult Patients In Addis Ababa
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Date
2006-07
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Addis Ababa Universty
Abstract
A study involving the Collection, Isolation, Identification and Sensitivity screening of major
bacterial uropathogens and also the antibacterial activity of two traditionally used plants against
the isolates of these uropathogens for various ailments in Ethiopia was conducted at Ethiopian
Health and Nutrition Research Institute (E1-INRI). The specimens for the isolation of the bacterial
uropathogens were collected from adult out·patients with clinically proved symptomatic Urinary
tract infections and referred to the EHNRI Reception of the Laboratory Core Service and the
Arsho Medical Laboratory by physicians from various Hospitals or Clinics. Out of the total 241
clean catch mid stream urine samples 66 were revealed to have significant bacteriuria. The most
frequently isolated species was E.coli (77.3%) followed by Klebsiella spp. (12.1 %),
PseudoJ/lonas .ljJjJ. (4.5%), Proteus spp. (4.5%) and En/erobac/er sjJjJ. (1.5%). In this study, high
level multidrug resistance was observed particularly for commonly prescribed antibiotics:
Amoxycillin (69.7%), Ampicillin (64.6%), Tetracycline (53.0%) and Co·trimoxazole (43.9%)
. using disc diffusion method. The ill Vi/I'D antibacterial activities of 80% methanol crude extracts
and their hydro alcoholic solvent fractionates prepared from both seeds of Albizia gl//JIl1lijera and
Albizia anli1elminlica were tested for their antibacterial activity against the clinical isolates of
E.coli (31), Klebsiella spp.(8), Pselldollionas spp.(3), Prole liS ~pp.(3) and also standard reference
strains of E.coli ATCC,25922 and P.aerllginosa ATCC,27853 using agar dilutiol) method. The
chloroform fraction didn't exhibit any activity while n·butanol fraction was found to have strong
antibacterial effects as compared to the aqueous solubilized fraction which showed antibacterial
activity only against E.coli and Klebsiella .Ipp. at I OOO~lg/m1. Minimum inhibitory concentration
values of n·butanol fraction against the clinical isolates of E.co/i showed stronger activity than
the commonly used broad spectrum antibiotics: Tetracycline ancl Amoxycillin. Additionally, a
beller activity was exhibited by this n·butanol fraction against the clinical isolates of Klebsiella
spp. and Pseudomonas ~pp. than Amoxycillin. Over all, in this study it was found that n·butanol
fraction of 80% methanol crude plant extracts of both plants may contain bioactive compounds
with therapeutic activity.
Keywords: Albizia al1li1ellilinlica, Albizia gllllllllifera, MIC, Uropathogenic E.coli, UTI
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Keywords
Albizia Anthelmintica, Albizia gummifera, MIC, Uropathogenic E.coli, UTI