Effect of Green Tea (Camellia Sinensis) Leaf Extract on Highly Active Anti- Retroviral Therapy Induced Dyslipidemia and Non Alcoholic Fatty Liver Disease in albino Wistar Rats

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Addis Ababa University


Back ground: Tea is the second widely consumed drink in the world next to water and used by people in different parts of the world for treatment of different health ailments; especially, green tea. HAART which is a recently(since 1996) developed form of ART medication to treat HIV/AIDS is now becoming hepatotoxic to patients and found to be more hazardous to the health of HIV patients. Objective: The aim of this study was to investigate the possible remedial effect of GTE on HAART induced derangement of lipid metabolism in albino Wistar rats. Materials and Methods: Thirty female albino Wistar rats 10-12 weeks old and weight of 145-200 grams were divided in to five groups of six each randomly. Group-I (normal control group) which were given 1mL of distilled water per day, Group-II (negative control group) which were given HAART only, Group-III (which were given HAART and 100mg/Kg of GTE), Group-IV (which were given HAART and 200mg/Kg of GTE) and Group-V (which were given HAART and 400mg/Kg of GTE) for sixty days. On the 60th day of the experiment rats was fasted overnight and sacrificed by cervical dislocation after giving di-ethyl ether general anesthesia. Then blood was taken by cardiac puncture for lipid profile investigation and for liver function test. Liver was also taken from each experimental rat for histopathological evaluation and triglyceride extraction. Results: The results of our experiment showed that, the body weight change showed a significant decrement (p<0.05) in group-II and more decrement in group-IV and group-V rats (p<0.01). AST and ALT level in group-V showed a significant decrement (p<0.05) but no significant change on serum ALP level though there is reduction. Serum lipid profile TC, TG and LDL-c showed a significant decrement (p<0.05) in group-V but no significant change in HDL-c in group-V. TTG (Tissue triglycerides) also showed a significant decrement (p<0.05) in group-V as compared to group-II. As demonstrated by NAS, most of group II rats developed steatosis followed by group-III rats and the histopathological observation showed the better remedial effect of GTE on liver especially with the higher dose (400mg/Kg). Conclusion: The results of this study demonstrated that the green tea leaf extract has a good protective effect against HAART induced hepatotoxicity.Hence,it prevents dyslipidemia and NAFLD which might be due to its antioxidant property. KeyWords;Highly Active Anti-Retroviral Therapy, Hepatotoxicity, Green Tea leaf Extract, Dyslipidemia, Non Alcoholic Fatty Liver Disease



Highly Active Anti-Retroviral Therapy; Hepatotoxicity; Green Tea leaf Extract; Dyslipidemia, Non Alcoholic Fatty Liver Diseas