Determination of Some Heavy Metals in two Selected Medicinal Plants Collected from Sidama Region in Masincho
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Date
2024-09-12
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Addis Ababa University
Abstract
In this study the collected samples of P. vogeliifruitsand R. myricoides leaves were cleaned, dried in an oven and then ground topowder form. Then 0.5gof each powdered sample was digested with 4 mLof 69.72%ofHNO3 and 1 mLof 70% of HClO4at a temperature of 300℃ for 4:00hrsand 3mLof 69.72% HNO3 and 1 mLof 70% HClO4at a temperature of 300℃ for 3:00 hrs respectively. An efficient wet digestion procedure with the percentage recoveries which range from 99.33-99.97% forP. vogelii and 94.7-98.9% for R. myricoides were developed. The contents of heavy metals (Cu, Cr, Cd, Zn and Pb) in P. vogelii and R. myricoides samplesweredetermined using FAAS and the result of mean concentrationsof these metals were compared with the limit values set by WHO. The mean concentrations of the selected heavy metals were determined from triplicate samples of P. vogelii and R. myricoides herbal medicinal plantswhichwere grown in Masincho area near Yirgalem town of Sidama region.In the present study, the mean concentrations of selected heavy metals analyzed inP. vogelii and R. myricoides samplesin mg/kg were Zn (75.23± 0.038), Cu (17.68± 0.023), Pb (10..66± 0.0019),Cd (3.15± 0.0066) and Cr (BDL);and Zn (10.50± 0.0098), Cu (34.98± 0.006), Cd (8.70 ±0.0063), Pb (18.60 ±0.0072) and Cr (BDL) respectively. The contents of chromium were too lowto be measured in both sampleswhich werebelow detection limit and therefore,were not reported.The levelsof theseanalyzed metals in this study are both higher and lower just likethe concentrations of metals reported indifferent literatures in medical plant fruits and leaves.
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P. Vogelii, R. Myricoides, Heavy Metals, World Health Organization and Flame Atomic Absorption Spectroscopy