Optimization of Bio-Paint Production Using Aspergillus Niger Under Liquid State Fermentation
| dc.contributor.advisor | Solomon Kiros (PhD) | |
| dc.contributor.author | Ejigayehu Desalegn | |
| dc.date.accessioned | 2025-01-14T13:58:36Z | |
| dc.date.available | 2025-01-14T13:58:36Z | |
| dc.date.issued | 2022-10 | |
| dc.description.abstract | Synthetic dyes are hazardous to living organisms and the environment in general. This has progressively led to an increased demand for natural colorants. The fungus kingdom has been extensively researched as a source of bioactive chemicals with many industrial applications. However, in Ethiopia, such studies are lacking. In this work, the synthesis of natural pigments by Aspergillus niger spp. in a submerged fermentation system was investigated. The species were obtained from the biodiversity institute and grown on PDA agar for 5-7 days at a temperature of 30 °C. Even if many factors influence pigment production from microbes, in this study, only pH (3–8), maltose concentration (0.024–0.072 g), and fermentation time (7–28 days) were examined. For pigment production, cultivation of Aspergillus niger spp. was carried out in potato dextrose broth supplemented with a carbon source (maltose) for a day and pH adjusted in shaking conditions (centrifugal force of 150 rpm). Natural paint ingredients were used with fungal dyes to produce bio-paint. To establish optimal cultural conditions, Design Expert version 13 was used. To determine the optimal setting, a Box-Behnken design under response surface methodology was used. A second-order polynomial equation was fitted to the data using multiple regression analysis. A UV-Vis absorbance measurement was employed to quantify the pigment concentrations. Thin layer chromatography and FT-IR spectroscopy were employed to molecularly characterize the pigment. In this study, the maximum pigment concentration of 357.425 a.u. was obtained for the optimized values of pH, maltose concentration, and fermentation time of 7.678, 0.045 g, and 27.953 days, respectively. The bio-paint was found to have values of pH, density, viscosity and drying time of 6.8 + 0.57, 1.3272 + 0.04mg/ml, 55 mPas + 1.34 and 30 min + 1.63 min, respectively, and demonstrated comparable properties with synthetic paints. When applied to a wall, the bio-paint showed remarkable color and coating performance. Fungal pigments could, therefore, be utilized in expanding bio-colored industries in Ethiopia. | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/4100 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Ababa University | |
| dc.subject | Pigment | |
| dc.subject | Fungi | |
| dc.subject | Aspergillus niger | |
| dc.subject | Submerged fermentation | |
| dc.subject | Paint | |
| dc.subject | RSM | |
| dc.subject | Optimization | |
| dc.title | Optimization of Bio-Paint Production Using Aspergillus Niger Under Liquid State Fermentation | |
| dc.type | Thesis |