Effects of Sucrose Concentration, Ph and Ms Media Strength on in Vitro Propagation of Oreosyce Africana (Hook F.) from Shoot Tip Explant
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Date
5/5/2020
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Addis Ababa University
Abstract
Oreosyce africana Hook. f. is herbaceous slender and climbing herbaceous plant that belongs to family Cucurbitaceae, a probably monotypic genus found in tropical Africa. The extract of this plant is found to be very efficient in killing mosquitoes which could be important to control mosquitoes. However, as this plant is endangered, it can be propagated by developing efficient micropropagation protocol. For optimization of the protocol, investigation of different factors that affects the explant in culture is important. Therefore, the objective of this study was to investigate the effect of sucrose concentration, pH and MS salt strength on micropropagation of O. africana. Shoots were initiated on MS medium containing 0.5 mg/l BAP and then transferred to different salt strength MS medium (1/4, 1/3, 1/2, full and double full MS) containing different concentrations of sucrose (3%, 2%, 1.5%, and 1%), 0.5 mg/l BAP at different pH (5.0, 5.4, 5.8, 6.2 and 6.6) for shoot multiplication. The multiplied shoots were transferred to half salt strength MS medium containing different concentrations of IAA and IBA for rooting followed by acclimatization. The maximum mean shoot numbers per explant (31.33) was obtained on full salt strength MS medium containing 3% sucrose at 5.8 pH. The highest mean number of leaves (81.03) and the longest mean length of shoot (12.99 cm) per explant were obtained on full strength MS medium after three weeks of culture. The maximum mean root number (23.80) was obtained on ½ MS medium containing 1mg/l IAA and 0.5mg/l NAA after thirty days of acclimatization, 83.33% of plants survived. O. africana is too much delicate herb plant because of this it is sensitive to hormone, pH and MS strength. Therefore, appropriate amount of MS strength, pH conditions, and optimum growth regulator should be used and also more emphasis is needed for it to multiply successfully. The protocol optimized can be used for mass propagation and improvement and conservation of O. africana.
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Salt, Strength, Sucrose, Ph and O, Africana, Micropropagation, In Vitro Propagation