Isolation of Pathogenic Bacteria and Antimicrobial Profiling Patterns Among Patients with Diabetic Foot Ulcer in Selected Hospitals in Addis Ababa, Ethiopia.
| dc.contributor.advisor | Dr. Yimtubezinash Woldeamanuel | |
| dc.contributor.advisor | Habtamu Biazin | |
| dc.contributor.advisor | Dr. Abdurezak Ahmed | |
| dc.contributor.author | Atlaw Asegdew | |
| dc.date.accessioned | 2021-10-29T07:07:22Z | |
| dc.date.accessioned | 2023-11-05T09:35:48Z | |
| dc.date.available | 2021-10-29T07:07:22Z | |
| dc.date.available | 2023-11-05T09:35:48Z | |
| dc.date.issued | 2021-08 | |
| dc.description.abstract | Background: Infected diabetic foot ulcer (IDFU) is one of the challenging problems globally, associated with diabetes mellitus. It is a leading cause of lower extremities amputation, sepsis, and even mortality. Both gram-negative and gram-positive bacteria including anaerobic bacteria and fungi are considered potential causes of infections. Objectives: This study aimed to isolate potential pathogenic bacteria and their antimicrobial susceptibility pattern including phenotypical screening of extended-spectrum betalactamases (ESBLs) and carbapenemase-producers among IDFU in selected hospitals in Addis Ababa, Ethiopia. Materials and Method: Institutional based cross-sectional study was conducted from November/2020 to May/2021 in selected hospitals in Addis Ababa, Ethiopia. A sterile swab was used to collect samples from foot ulcers while a sterile needle was used to collect pus aspirates. All isolates were identified by using routine bacterial culture, Gram-staining, and a panel of biochemical tests. For each identified bacterial species, antibiogram profiles were determined by the Kirby Bauer disk diffusion method based on the Clinical and Laboratory Standards Institute guidelines. Double disk and modified carbapenem (meropenem) inactivation methods were used for screening ESBL and carbapenemase based on CLSI 2020 guidelines. Results: Out of the 130 recruited participants, 88 (68.2%) were males and 42 (33%) were females. One hundred twenty-seven pathogenic bacteria were isolated, of which, the predominant bacteria was S.aureus 25.19% (32/127), followed by Pseudomonas species 18.89% (24/127). Gram-positive isolates were susceptible to Chloramphenicol, Clindamycin, and Amikacin. Gram-negative isolates were also sensitive to chloramphenicol, Aztreonam, and Amikacin. Overall, 92.9% (118/127) were multidrug-resistant. From the gram-negative bacteria, 53.94% (41/76) were extended-spectrum beta-lactamases (ESBLs) producers, and 26.47% (19/68) were carbapenemase-producers. Conclusion: Bacteria that infect diabetic foot ulcers are multidrug-resistance. The results emphasize the importance of identification of the causative agent and antimicrobial susceptibility testing before the selection of appropriate antibiotic(s) to treat diabetic foot ulcers. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/28397 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Diabetic Mellitus, Diabetic foot ulcer, Antimicrobial testing, Multidrugresistance, ESBLs, Carbapenemase-producers, Addis Ababa, Ethiopia. | en_US |
| dc.title | Isolation of Pathogenic Bacteria and Antimicrobial Profiling Patterns Among Patients with Diabetic Foot Ulcer in Selected Hospitals in Addis Ababa, Ethiopia. | en_US |
| dc.type | Thesis | en_US |