Evaluation of Wound Healing Activity of 70% Ethanol Leaf Extract of Becium grandiflorum Lam. (Lamiaceae) in Mice
| dc.contributor.advisor | Shibeshi, Workineh(PhD) | |
| dc.contributor.advisor | Engidawork, Ephrem (PhD) | |
| dc.contributor.author | Beshir, Kald | |
| dc.date.accessioned | 2018-06-21T23:18:07Z | |
| dc.date.accessioned | 2023-11-06T08:08:03Z | |
| dc.date.available | 2018-06-21T23:18:07Z | |
| dc.date.available | 2023-11-06T08:08:03Z | |
| dc.date.issued | 2017-01 | |
| dc.description.abstract | Based on traditional claims, leaves of Becium grandiflorum were investigated for their potential wound healing activity using excision and incision wound models. In addition histological analysis, antiinflammatory and antibacterial activity of the leaves was performed. Wound healing activity was studied by topical application of simple ointment of the leaf extract at a concentration of 5% and 10% (w/w) after it was extracted by maceration using 70% ethanol. Toxicity of the formulated ointments was studied by skin irritation test on Swiss albino mice. For antiinflammatory study, carrageenan-induced hind paw edema model was used at a concentration of 100, 200 and 400 mg/kg. The antibacterial activity of the extract was examined using disk diffusion technique against several pathogenic bacterial strains that are commonly occurred in wound at a concentration of 50, 100 and 200 μg/μl. The ointment formulation of the extract was found to be non-irritant at 5% concentration. However, continuous application of 10% showed skin irritation. Treatment of wound with the ointments exhibited significant (p< 0.001) increase in wound contraction rate, shorter epithelization time, higher skin tensile strength that was supported by considerable deposition of collagen, fibroblast proliferation and vascularization form histological analysis. The plant extract also showed significant (p<0.001) inhibition of inflammation and antibacterial activities, that could be a mechanism of wound healing. From the results obtained, it can be concluded that the 70% ethanol extract of B. grandiflorum enhance wound healing activity most probably via its higher collagen deposition, antiinflammatory and antibacterial effects, supporting the traditional use of this plant as wound healing agent. Key words: Wound healing, Excision model, Incision model, Histophatological analysis, Antiinflammatory, Antibacterial, B. grandiflorum. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/2820 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Wound healing; Excision model; Incision model; Histophatological analysis; Antiinflammatory; Antibacterial;B. grandiflorum | en_US |
| dc.title | Evaluation of Wound Healing Activity of 70% Ethanol Leaf Extract of Becium grandiflorum Lam. (Lamiaceae) in Mice | en_US |
| dc.type | Thesis | en_US |