Pathogenic Escherichia Coli Strains and their Antibiotic Susceptibility Profiles In Cases of Child Diarrhea at Tikur Anbessa General Specialized Hospital, AAU College of Health Sciences, Addis Ababa, Ethiopia.
No Thumbnail Available
Date
2018-05-03
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
The prevalence and antibiogram of pathogenic E. coli strains which cause diarrhea vary from
region to region, and even within countries in the same geographical area. In Ethiopia, diagnostic
approaches to E. coli induced diarrhea in children less than five years of age are not
standardized. The aim of this study was to determine the involvement of pathogenic E. coli
strains in child diarrhea and determine the antibiograms of the isolates in children less than 5
years of age with diarrhea at Addis Ababa University College of Health Sciences Tikur Anbessa
General Specialized Hospital, Addis Ababa, Ethiopia. Children (n=98) (47 males & 51 females),
aged 01 month to 60 months suffering from diarrhea were included in this study and stool
specimens were collected and processed from January 2017 to March 2018. Fecal specimens
were inoculated on MacConkey and Eosin Methylene Blue agar to grow E. coli. E. coli isolates
were biochemically confirmed using biochemical tests indole, methylred, Vogues proskuer and
citrate. Antimicrobial susceptibility profiles were determined using Kirby Bauer disk diffusion
method. Heating method was used to extract DNA from E. coli isolates. 58 E. coli isolates were
detected out of which 25 pathotypes belonging to different classes of pathogenic strains: STEC,
EPEC, EHEC, EAEC were detected by using PCR technique. Pathogenic E. coli exhibited high
rates of antibiotic resistance to many of the antibiotics tested. Moreover, they exhibited multiple
drug resistance. The involvement of antibiotic resistant pathogenic E. coli in diarrheic children
was prominent and hence focus should be given on the diagnosis and antimicrobial sensitivity
testing of pathogenic E. coli at AAU CHS Tikur Anbessa General Specialized Hospital. Among
antibiotics tested, Cefotitan could be a drug of choice to treat E. coli.
Description
Keywords
Pathogenic, Escherichia Coli, Antibiotic Susceptibility, Cases of Child, Tikur Anbessa General Specialized Hospital