Molecular Epidemiology and Drug Sensitivity of Mycobacterium Tuberculosis Isolates Among New Pulmonary Tuberculosis Patients in Arsi Zone Oromiya Region, Ethiopia
| dc.contributor.advisor | Abebe, Tamrat(PhD) | |
| dc.contributor.advisor | Mihret, Adane(PhD) | |
| dc.contributor.author | Tamrat, Fekadu | |
| dc.date.accessioned | 2018-06-29T13:52:17Z | |
| dc.date.accessioned | 2023-11-05T09:35:52Z | |
| dc.date.available | 2018-06-29T13:52:17Z | |
| dc.date.available | 2023-11-05T09:35:52Z | |
| dc.date.issued | 2016-10 | |
| dc.description.abstract | Back ground: - The 22 high burden countries accounts 80% of global tuberculosis burden, and Africa alone account 28% of Global TB burden where Ethiopia is at 16th from the world, and 9th from the continent accounting an incidence of 200 cases per 100,000 population according to WHO 2015 Tuberculosis report. According to WHO Global TB report, Ethiopia is ranked 15th among 27 high burden MDRTB and XDR-TB countries. The estimated MDR-TB from new cases was 3.3% according to WHOGTR 2015. Specifically in Arsi Zone, PTB+ case notification in the past fifteen years, from 1997 to 2011 increased from 6.9 to 63 per 100,000 populations. Objective: The objective of this study was to characterize the diversity of Mycobacterium tuberculosis strains circulating and to determine the drug susceptibility of the isolates among new PTB+ patients in Arsi zone, Oromiya region, Ethiopia. Methods: - A cross-sectional study design using quota sampling technique was under taken. All smear positive samples were collected from seven selected health facilities which were stated having higher prevalence TB cases in the zone and parallely, questionnaire was filled for socio demographic analysis. Culture on conventional Löwenstein-Jensen (LJ) media and drug sensitivity test using Line Probe Asay (LPA) was done at Adama Regional Laboratory (ARL). Spoligotyping was done in Aklilu Lemma Institute of Pathobiology (ALIPB), AAU and the isolates were compared with the SpoIDB4 database of the Pasteur Institute of Guadeloupe. Results: A total of 150 smear positive pulmonary TB patients were included in this study of which 130 samples were grown on LJ media. The study participant’s age range was from 6 -72 years with the mean of 29 and (±SD) of 13.25, of which 10 % (15/150) of the study participants were below the age of 15.Molecular characterization showed that104/130(80.0%) were Euro-American, 15/130 (11.5%) Indo-Oceanic, 9/130 (6.9%) Unknown lineage, 1/130(0.8%) West African-1 and 1/130(0.8%) Mycobacterium bovis. Drug Sensitivity Test on Line Probe Assay showed that 2 (1.53%) isolates were mono resistance for Rifampicin, 5 (3.8%) mono resistance for INH, and 3 (2.3%) were resistant for both INH and Rifampicin. Prevalence of MDR from New patients was 5/130 (3.8%). Conclusion: - In this study we have shown that the prevalence of MDR-TB from the new TB patients is 3.8% which is high, and the type of the TB lineage circulating dominantly is Euro-America from which the family of “T” is the most circulating strain indicating that there is a rapid circulation among the society. Keywords: - Mycobacterium tuberculosis, Molecular epidemiology, DST, spoligotyping | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/5131 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Mycobacterium tuberculosis | en_US |
| dc.subject | Molecular epidemiology | en_US |
| dc.subject | DST | en_US |
| dc.subject | Spoligotyping | en_US |
| dc.title | Molecular Epidemiology and Drug Sensitivity of Mycobacterium Tuberculosis Isolates Among New Pulmonary Tuberculosis Patients in Arsi Zone Oromiya Region, Ethiopia | en_US |
| dc.type | Thesis | en_US |