Isolation and Characterization of Aerobic Cellulose-Degrading Microorganisns
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Date
1983-06
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Addis Ababa University
Abstract
Seventeen species of cellulose-degrading fungi that
fall into fifteen genera were isolated from decomposing
plant matter, p;arden soil, arable soil and manure. They
were Alternaria sp., Aspergillus ilayus, ~. niger,
Cephalospor:ul!', sp., Chaetomium sp., Curvularia sp., Fusarium
sp., Geotrichum sp., Helminthosporium sp., Penicillium sp.,
Pythium sp" Rhizopus sp., Stachbotrys sp., Trichoderml.!.
sp. 1, Trichoderma sp. 2, Verticillium sp. and
Zygorrhynchus sp.
Gr01,th rate determination on filter 1)aper showed that
A. niger, Chaetomium sp., ~urvularia sp., Helminthosporium
sp., Rhizopu~ sp., Stachybotr12 sp., Trichoderma.
sp. I and 2 and Zygorrhynchus sp., displayed abundant
growth within 7-21 da~at pH 4.5, 7.2 and B.S.
Out of the fungi tested, only eight isolates namely
Cephalosporium sp., Curvularia sp., HelminthosporiuJ!l: sp.,
Pythium sp., Stachybotrys sp., Trichoderma sp. 2.,
Verticillium sp., and Zygorrhynchu~ sp., were found to
elaborate free cellulase in the culture medium.
High cellulase activity was found in Zygorrhynchus sp.
at pH 7,2 and Stachybotrys sp., at pH 4.5. Low
cellulase activity was shown by Cephalosporium sp.,
at pH 7.2 and Pythium sf., at pH II,S The effect of different cellulosic substrates on
cellulase elaboration was tested on these organisms.
The influence of cellobiose and glucose on the
in vitro cellulase activity of ~ygorrhynchus sp.,
was tested. The type and concentrations of reducing
sugars released in the enzyme hydrolysates of the
eight species were evaluated.
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Biology