Isolation and in-Vitro Characterization of Extracellular Phytase Producing Bacterial Isolates for Potential Application in Poultry Feed
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Date
2020-07-07
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Addis Ababa University
Abstract
Phytase is an enzyme that can be breaking down the complex organic forms of phosphorous such as phytate into simpler forms by sequential hydrolysis of phosphate ester bonds and liberate the usable form of inorganic phosphates. Supplementation of feeds with microbial phytase enhances the bioavailability of phosphorus and micronutrients. Hence, the aim of this study was to isolate and characterize phytase producing bacteria from Rhizospheresoil, fresh poultry excreta, Cattle shed in order to evaluate their potential in improving poultry feeds. Phytase producing bacterial were isolated using Wheat Bran Extract Medium. A total of 169 bacterial isolates were purified and screened for phytase activity. Out of which36 were found positive for the desired purpose. The bacterial isolates were identified by cultural, morphological, and biochemical features. Finally, the isolates were identified to species level by 16S RNA genes sequencing using universal primers. The isolate (RS1, RS8, RS10 and RS15) correlated with shared the highest similarity indices, Enterobacter asburiae, Klebsiella pneumonia, Pseudomonas aeruginosae and Escherichia coli respectively. all isolates alone possible to increase the phytase production in cultures medium (wheat bran extract medium) by supplementing with 1% galactose and ammonium sulphate, temperature (50oC), pH (6), incubation time (72 hr) and size of inoculum (at 800μl). Proximate composition analysis after supplementation of phytase released the bound phytate phosphorus and improved bioavailability of phosphorus, calcium, potassium and sodium in poultry feed. Overall, this study is to improve the nutritional value of poultry feed, reducing the cost of diets and supplementation inorganic phosphate. Lastly, it will be maximizing poultry products by supplementation of phytase with poultry feed.
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Phytase, Rhizosphere Soil, Wheat Bran, Industries and Poultry