Hepatoprotective Effect of Silymarin on Fructose Induced Nonalcoholic Fatty Liver Disease in Male Albino Wistar Rats
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Date
2016-06
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Addis Ababa University
Abstract
Background: Nonalcoholic fatty liver disease is one of the most common causes of
chronic liver disease in the Western world, and it‟s likely to parallel the increasing
prevalence of type 2 diabetes, obesity, and other components of metabolic syndrome.
There is also growing evidence in both animal models and human studies suggesting that
high dietary intake of fructose is an important nutritional factor in the development of
metabolic syndrome and its associated complications of NAFLD. However, an optimal
parameters in the fructose model group had been partially ameliorated when treated with
silymarin.
Conclusion: The rats fed with fructose developed dyslipidemia, oxidative stress and mild
steatosis in liver, which are the characteristics feature of nonalcoholic fatty liver disease.
When treated with silymarin showed amelioration in oxidative stress, dyslipidemia and
steatosis of liver in some extent but could not reach the normal control values.
Key Words: Nonalcoholic Fatty Liver Disease, Silymarin; Lipid Peroxidation;
Dyslipidemia; Total Antioxidant Status; Reduced Glutathione.
treatment for NAFLD has not been established. Silymarin is gaining increasing
recognition due to its beneficial effects in the control and prevention of alcoholic liver
disease, acute and chronic viral hepatitis and toxin-induced liver diseases.
Objective: The current study aim to investigate the hepatoprotective effect of silymarin
on fructose induced nonalcoholic fatty liver disease in rats.
Methodology: Thirty male Wistar rats were randomly divided into five groups: normal
control group that consumed tap water; Silymarin control group that consumed tap water
+ silymarin (400 mg/kg/day), fructose control group that consumed 20% fructose
solution; Treatment group that consumed 20% fructose solution + Silymarin (200
mg/kg/day) and treatment group that consumed 20% fructose solution + silymarin (400
mg/kg/day). The food and liquid intake, body weight, liver triglyceride, serum lipid
profile, lipid peroxidation, antioxidant level and morphological as well as
histopathological changes of liver were investigated. The data were analyzed using one
way analysis of variance (ANOVA) followed by tukey multiple comparison test and
statistical significance was determined at p ˂ 0.05.
Result. This study showed that the fructose consumed model group had a significantly
high values in the stage of steatosis grade, liver weight, hepatic triglyceride, serum
triglyceride, total cholesterol, low density lipoprotein cholesterol, alanine amino
transferase, aspartate aminotransferase and hepatic malondialdehyde concentration as
compared to the normal control and contrariwise a significantly low values of reduced
glutathione, plasma total antioxidant capacity and food consumption. The altered
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Keywords
Nonalcoholic Fatty Liver Disease; Silymarin; Lipid Peroxidation; Dyslipidemia; Total Antioxidant Status; Reduced Glutathione