Investigation on the Efficacy of Crude Root Extract of Cucumis Ficifolius A., Rich on Selected Biochemical and Histopathological Parameters in High-Fat-Fructose Diet-Streptozotocin-Induced Type-2 Diabetic Albino Wistar Rats
| dc.contributor.advisor | Seifu Daniel | |
| dc.contributor.author | Ayele Yeshambel | |
| dc.date.accessioned | 2021-01-10T08:22:01Z | |
| dc.date.accessioned | 2023-11-29T04:18:24Z | |
| dc.date.available | 2021-01-10T08:22:01Z | |
| dc.date.available | 2023-11-29T04:18:24Z | |
| dc.date.issued | 2020-09 | |
| dc.description.abstract | Background: Diabetes mellitus, mainly T2DM (type-2 diabetes mellitus), has resulted in large scale and exponentially increasing burdens in health and socioeconomic aspects of various populations worldwide. The plant Cucumis ficifolius A., Rich root parts have been used for treatment of diabetes by traditional medical practitioners of Ethiopia. Objective: This study was aimed to validate the efficacy of the crude 80% methanol extract of roots of C. ficifolius on blood glucose, pancreatic islets of Langerhans β-cells, lipid profile, body weight, and liver and kidney parameters on induced type 2 diabetic rats. Method: The crude 80% methanol extract of roots of C. ficifolius was prepared using maceration technique. Phytochemical screening (qualitative) and acute toxicity of the crude extract were determined. T2DM rat model was developed by feeding “High-Fat-Fructose Diet” followed by induction of single 30mg/Kg dose Streptozotocin. Total of 6 groups (n=7) of albino wistar rats: three control groups (normal control, diabetic negative control and diabetic positive control (metformin 100mg/kg) groups) and three experimental groups (diabetic+ crude C. ficifolius root extract at 100, 200 & 400mg/kg doses) were used. The effect of crude root extract on blood glucose, body weight, fasting serum lipid profile, histo-pathology of pancreatic islets of Langerhans; liver and kidney biochemical and histo-pathological parameters were determined. Data analysis was done by SPSS version-24 using ANOVAs. Result: From crude 80% methanol extract of roots of C. ficifolius terpenoids, flavonoids, saponins, glycosides, phenols, alkaloids and steroids were present. There was no rat death and toxic sign at 2000mg/Kg acute test. Against diabetic negative control group, those groups treated with crude plant extract showed dose dependent: a) blood glucose reduction which was moderate in STT, continuing in progressive tests and significant . Key words: C. ficifolius, traditional medicine, anti-diabetic activity, type-2 diabetes mellitus | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/24585 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Abeba University | en_US |
| dc.subject | C. ficifolius, traditional medicine, anti-diabetic activity, type-2 diabetes mellitus | en_US |
| dc.title | Investigation on the Efficacy of Crude Root Extract of Cucumis Ficifolius A., Rich on Selected Biochemical and Histopathological Parameters in High-Fat-Fructose Diet-Streptozotocin-Induced Type-2 Diabetic Albino Wistar Rats | en_US |
| dc.type | Thesis | en_US |