Enzymatic Determination of Urea at the Membrane-Stabilized Liquid-Liquid Interface
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Date
1999-06
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Addis Ababa Universty
Abstract
The membrane-stabilized water-nitrobenzene interface was used as an
amperometric (or voltammetric) ammonium ion sensor for an indirect detection of
urea. The ammonium ion was produced by the action of watermelon seeds and soy
bean flour on urea in the aqueous phase (5 mmol/L Li2S04). The detection was
based on the facilitated transfer of ammonium ions by DB[18JC-6. The organic phase
contained 50 mmol/L DB[18JC-6 and 10 mmol/L EthVTPB. The performance of
watermelon seeds and soy bean flour as soluble urease reagents is described. The
problem caused by potassium indigenous to the tissues is discussed. The sensor
was applied to the estimation of potassium in the seeds of some plants
Description
Keywords
Liquid-Liquid Interface