Isolation and Molecular Characterization of Lumpy Skin Disease Virus in Central Ethiopia
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Date
2021
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Addis Ababa University
Abstract
Lumpy skin disease (LSD) is high impact viral disease affecting cattle in various parts of Ethiopia and considerable number of countries worldwide. Taxonomically, LSD virus is classified in the family Poxviridae of the genus Capripoxvirus. The disease is caused by LSD virus and is characterized by nodules on the skin that cause permanent damage to hides and skins. LSD a serious disease that has been impossible to control in Ethiopia which is made clear by the outbreak that occurs year in and year out. Even though a continuous work has been done to understand the disease, tackling it prove to be unrealizable, urging more work to be done as much as possible that can assist as a building block to bring this devastating disease to its annihilation. The current work hopes to do that by isolating and characterizing of the virus that cause this disease from the most recent outbreak incident. A purposive sampling technique was mplemented in the town of Mojo, Ejere, Ejersa and koka. Cell line originated from sheep skin (Embryonic skin of sheep/ESH-L), highly sensitive to Capripoxvirus, was used for the isolation of the infectious virus. The isolates were further processed for classical and real time PCR in order to genotype. The virus that was detected as LSDV, have been further characterized through the RPO30 gene amplification for sequencing and phylogenetic tree construction in relation to different viral isolates from a previous work done though out the country and beyond. Out of 15 samples collected 10 of them were found to be positive for LSD. Further sequencing shows there was a two nucleotide position variation when comparing the present study isolates and the vaccine (KS-1) resulting in a single nonsense amino acid mutation. Constant outbreak investigation and full gene sequencing are the major suggestion of the study.
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Keywords
Lumpy skin disease, LSDV, RPO30, molecular characterization, virus isolation