Production of Proteases by Alkalophilic Bacteria from a Hot Sprinq at Wondo Genet
| dc.contributor.author | Kassa, Atalo | |
| dc.date.accessioned | 2021-12-20T11:03:24Z | |
| dc.date.accessioned | 2023-11-08T16:34:04Z | |
| dc.date.available | 2021-12-20T11:03:24Z | |
| dc.date.available | 2023-11-08T16:34:04Z | |
| dc.date.issued | 1993-06 | |
| dc.description.abstract | Two organisms with high proteolytic activity on casein, gelatin or haemoglobin were selected out of the bacterial isolates obtained from a hot spring at Wondo Genet. The test organisms were Gram posi ti ve, rod-shaped, spore-forming with chains of two or more cells, motile bacteria. Based on the biochemical and morphological tests, it has been confirmed that they belong to the genus Bacillus. Growth of the two isolates was observed in the pH and temperature ranges of between 5-10, and 30-55 °C , respectively. Their optimum pH and temperature for growth were 10 and 55°C. Glucose 0.5% and peptone at 0.5%, 0.4%, respectively resulted better yield of biomass and protease production. 0.5mM Ca +2 , O.lmM Mg+2 , and 1mM P044 increased biomass and enzyme production of both isolates. High enzyme production was observed in the presence of 1mM Cu+2 and 5mM Mn+2 for Bacillus sp-A, and O.lmM Zn+2 and 5mM Mn+2 for Bacillus sp-B. The highest enzyme activity of both isolates was observed when assayed at 55°C and pH 9.5. The protease of Baccilus sp-A showed higher hydrolytic activity on casein than on gelatin or haemoglobin. The protease of Bacillus sp-B have been found to hydrolyse only gelatin. However, both isolates failed to hydrolyse egg albumin. The two organisms were more efficient in degrading skin when supplied in the growth medium than feather, horn or hair. T high degrading activity on feather than Bacillus sp-B. On the other hand Bacillus sp-B showed high degrading activity on horn than Bacillus sp-A. The protease of Bacillus sp-A was thermally stable between 60-70 o C, with a half life of 50 min at 60°C and 65°C, and 40 min at 70°C. The protease of Bacillus sp-B was thermally stable at 60 and 65°C, and the half life was 50 min at these temperatures. However, the enzyme activity of both isolates ceased after 60 min of incubation at the temperatures examined. viihe least weight lose was recorded on hair. Bacillus sp-A showed high degrading activity on feather than Bacillus sp-B. On the other hand Bacillus sp-B showed high degrading activity on horn than Bacillus sp-A. The protease of Bacillus sp-A was thermally stable between 60-70 o C, with a half life of 50 min at 60°C and 65°C, and 40 min at 70°C. The protease of Bacillus sp-B was thermally stable at 60 and 65°C, and the half life was 50 min at these temperatures. However, the enzyme activity of both isolates ceased after 60 min of incubation at the temperatures examined. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/29356 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa,University | en_US |
| dc.subject | Production of Proteases by Alkalophilic Bacteria | en_US |
| dc.title | Production of Proteases by Alkalophilic Bacteria from a Hot Sprinq at Wondo Genet | en_US |
| dc.type | Thesis | en_US |