Poultry Producers‟ Knowledge, Attitude and Practices Regarding Antibiotics Use and Detection of Multi-Drug Resistance Profile of Escherichia Coli from Selected Farms in Bishoftu Town, Central Ethiopia
No Thumbnail Available
Date
2023
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Abeba University
Abstract
Antibiotics are used at sub_ therapeutic dose for growth promotion and prohylaxis in poultry are now on the rise which predisposes to the development and transfer of anti biotics resistance .A cross_ sectional study was carried out to assess the knowledge, at titudes and practice of poultry producer regarding antibiotics use and resistance using semi_ structured questionnaire to selected poultry farms in Bishoftu town, Ethiopia. Ei ghteen poultry farms were visited for sample collection to detect the resistance of E. coli from cloacal swab and litter samples using systematic random sampling, Identific ation of E. coli was carried out by MALDI_ TOF. Kirby bauer disk antimicrobial susce ptibility test method was conducted to determine antibiotics resistance of E. coli. Desc riptive tatistics, chis_ quare, and logistic regression were used to determined the assoc iation of explanatory variable with knowledge, attitude and practice, antibiotic usage
history, and resistance profile of E.coli. 61%, 67% and 56% of respondents in the stud y area had sufficient knowledge, good attitude and favorable practice on antibiotics us e and resistance, respectively. Knowledge of the usage of antibiotics and resistance was associated with respondents' age (> 45, OR = 3.6, p = 0.014), and farming experi ence (> 10, OR = 14.3, p = 0.00). The respondents' attitudes were also associated with their age (> 45, OR = 16, p = 0.001), education (Graduates, OR = 6.25, p = 0.007), and farming experience (> 10; OR = 5.4, p = 0.04). Practice of responders was associated with education level (Graduate, OR = 6, p = 0.01) and farming experience (>10, OR = 4.89, p = 0.004). According to correlation analysis, attitudes and the use of antibiotics as preventive medicine have a positive linear link (r = 0.81, p = 0.00), as well as attitudes and growth promotion (r = 0.47, p = 0.00). Additionally, there were s
ignificant positive correlations between the usage of prophylactic antibiotics (r = 0.81 5, p = 0.00) and the stimulation of growth (r = 0.592,p = 0.00). Tetracycline, Sulpham ethoxazole + trimethoprim and Sulfonamide were commonly used in the study farms. E. coli was confirmed in 46% of cloacal sample, andin eleven farms by MALDI_ TOF. 75% of the E. coli were showed multidrug antibiotisresistance. The study revealed co ncordance of farm antibiotic use purpose and resistance profile of the E. coli, warranti ng strict regulation to reduce antibiotics usage in poultry.