Blood and Tissue Enzyme Activities of Gdh, Ldh, Index of Glutathione and Oxidative Stress Among Breast Cancer Patients Attending Referal Hospitals of Addis Ababa, Ethiopia: A Comparative Cross Sectional Study
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Date
2017-05
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Addis Ababa University
Abstract
Background: The exact cause of breast cancer is still unknown, however, it is a multifactorial and
devastating disease. It is commonly diagnosed and second leading cause of death among women
globally. Scholars are searching for an early marker for diagnosis, prognosis and potential target
for treatment by looking it through many directions. One of the direction is its metabolism, all
types of cancer need high energy input and substrates via aberrant metabolism for their survival.
Therefore, the present study focused on investigating and examining pathways of glucose and
glutamate metabolism and oxidative stress in breast cancer patients via analysis of glutamate
dehydrogenase, lactate dehydrogenase, reduced and oxidized glutathione and the oxidative stress
index of breast cancer patients.
Methodology: Comparative cross-sectional study was conducted from July, 2015 to May, 2017.
A total of 54 (27 breast cancer patients and 27 control groups) participants were recruited. All were
age-sex matched and mean age of breast cancer patients were 44.93 (25–68), whereas mean age
of controls were 45.2 (24-66).
Result: Catalytic activities of glutamate dehydrogenase, lactate dehydrogenase and oxidative
stress index were significantly increased both in serum (4.2mU/ml, 78.6mU/ml and 3.3:1
respectively) and cancerous tissues (1.4mU/ml), 111.7mU/ml and 2.15:1 respectively) of breast
cancer patients as compared to serum of control groups (3.15mU/ml, 30.4mU/ml and 2.05:1
respectively) and non-cancerous tissue of breast cancer patients (0.92mU/ml, 70.5mU/ml and 1.1:1
respectively) (P≤0.05). Correspondingly, ratios of reduced to oxidized glutathione were
significantly decreased both in serum (20:1) and cancerous tissues (23.5:1) of breast cancer
patients when compared to serum of control groups (104.5:1) and non-cancerous tissues of breast
cancer patient (70.9:1) (P≤0.05).
Conclusion: Catalytic activities of glutamate dehydrogenase and lactate dehydrogenase, and ratios
of total oxidative status to total antioxidant capacity were significantly increased among breast
cancer patients when compared to serum of control groups and non-cancerous tissues of breast
cancer patients. While, ratios of reduced to oxidized glutathione and total antioxidant capacity in
serum and tumor tissues of breast cancer were significantly decreased as compared to serum of
control groups and normal tissues of breast cancer patients.
Key Words: Glutamate Dehydrogenase, Lactate Dehydrogenase, Reduced Glutathione, Oxidized
Glutathione, Total oxidative status and Total antioxidant capacity and breast cancer
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Keywords
Glutamate Dehydrogenase, Lactate Dehydrogenase; Reduced Glutathione; Oxidized Glutathione, Total oxidative status and Total antioxidant capacity and breast cancer