Evaluation of hypoglycemic effect of 80% methanol extract and solvent fractions of Crotonmacrostachyus leaves in streptozotocin- induced hyperglycemic mice
No Thumbnail Available
Date
2019-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Abeba University
Abstract
Diabetes Mellitus (DM) is a metabolic disorder characterized by hyperglycemia, which is usually
due to an absence of insulin, impaired effectiveness of insulin action or tissue insensitivity to
insulin. It can be classified into type I, type II and gestational DM. Its treatment is based on insulin
injection and oral hypoglycemic agents. However, rural parts of worldwide people with DM relay
on traditional remedies from plants sources with minimal side effects. This study was conducted
to evaluate hypoglycemic effect of 80% methanol extract and solvent fractions of Croton
macrostachyus leaves in normal, glucose loaded and Streptozotocin (STZ)-induced hyperglycemic
mice. 80% methanol extract and different solvent fractions of Croton macrostachyus leaves were
prepared. Sprague-Dawley mice of either sex were selected for the experiments. In this study mice
were grouped into five groups (six mice per group). Hyperglycemia was induced by single
intraperitoneal injection of STZ (135mg/kg body weight). Preliminary phytochemical screening
was done using standard procedures and acute toxicity study was done as per OECD 425
guidelines. The results were analyzed using one way ANOVA followed by post hoc test at 5%
level of significance. 80% methanol and solvent fractions of study plants have blood glucose
lowering effect on normoglycemic, glucose loaded and STZ-induced hyperglycemic mice at all
doses. However, mice that took chloroform fraction at dose of 100 and 200 mg/kg didn’t show any
significant reduction in blood glucose level on glucose loaded mice. In addition dose-dependent
reductions in blood glucose levels were observed in 80% methanol extract and solvent fractions.
Phytochemical screening indicated that alkaloid; flavonoids, tannins, phenols and sapponin were
presented in leaves extracts. It was also observed that the extracts didn’t have acute toxicity at a
single dose of 2 g/kg body weight of mice. So the traditional use of the study plant for the
management of DM can be supported as an alternative herbal supplement with further
investigation needed to make final conclusion.
Description
Keywords
Diabetes mellitus, Croton macrostachyus, Streptozotocin, Sprague-Dawley micenormoglycemic, oral glucose tolerance test, hyperglycemic