Alkaline Protease of Alkaliphiles Isolated from Ethiopian Rift Valley Soda- Lakes
| dc.contributor.advisor | Abate Dawit (PhD) | |
| dc.contributor.advisor | Gesesse Amare (PhD) | |
| dc.contributor.author | Hasana Azaga | |
| dc.date.accessioned | 2018-06-22T06:14:13Z | |
| dc.date.accessioned | 2023-11-08T16:39:06Z | |
| dc.date.available | 2018-06-22T06:14:13Z | |
| dc.date.available | 2023-11-08T16:39:06Z | |
| dc.date.issued | 2000-05 | |
| dc.description.abstract | A prntcolytic alkali phi lie bacteria was selected out of the bacterial isolates obtained from Riftvallcy soda lakes. The isolate was obtained from lake Chitu water sample. The organism was Gram variable aerobic, rod shaped spore forming, motile bacterium. It has been identified to belongs to the genus Bacillus. The growth of the isolate coded as CH-W 1 was observed in the pH and temperature ranges between 7-l l and 25-40°C respectively. Protease production was observed shortly afler inoculation reaching to maximum after 48 h. The crude enzyme had a temperature optimum of 55 °C and a pH optimum of 9. The enzyme was stable in a broad pH range of 8.5- l 0.5 after l hr incubation at 50 ° C . It has a half-life of 30 min. at 60°C . The enzyme was slightly activated by CuSO4 . Where as Ba++ ,Zn ++, Co++ , Hg++ , Mn ++, Fc+r ,.Na', K'and Ca" had very little.or no effect cin the activity of the enzyme. The enzyme was strongly inhibited by I mM PMSF sh o\,,ing that it belongs to the class of serine protease. EDTA at a concentration of IO mM partially inhibits the activity. This shows the requirement of Ca'' for stability. The organism was efficient in degrading Nug meal (GuizotaAbyssinia) and feather when used as a sole carbon source. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/2865 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Biology | en_US |
| dc.title | Alkaline Protease of Alkaliphiles Isolated from Ethiopian Rift Valley Soda- Lakes | en_US |
| dc.type | Thesis | en_US |