Entamoeba Species Commonly Diagnosed as Entamoeba Histolytica and Enteric Bacteria in the Pathogenesis of Diarrhea in Addis Ababa.
| dc.contributor.advisor | Petros Beyene (Professor) | |
| dc.contributor.advisor | Kebede Amha (PhD) | |
| dc.contributor.author | Worku Asnakew | |
| dc.date.accessioned | 2019-12-03T10:50:26Z | |
| dc.date.accessioned | 2023-11-08T16:33:48Z | |
| dc.date.available | 2019-12-03T10:50:26Z | |
| dc.date.available | 2023-11-08T16:33:48Z | |
| dc.date.issued | 2007-08-05 | |
| dc.description.abstract | Clinical reports relying on microscopic diagnosis give an impression that intestinal amoebiasis is very common in Ethiopia. However, recent species specific PCR-based method had shown little or no true infection with Entamoeba histolytica. The present study was conducted to assess the association of diarrheagenic bacteria vis-à-vis the Entamoeba trophozoites commonly diagnosed as E. histolytica in diarrheal patients. One hundred and fifty diarrheal patients from Selam Health Center in Gulelie Sub-city and Addis Ketema Propride Clinic in Addis Ababa were recruited in a study conducted between December 2005 and April 2006. Single stool samples were collected from the study participants, and inspected macroscopically for consistency, appearance, and the presence of gross blood. Wet mount of the stool samples was made to detect the presence of fecal leukocytes and red blood cells (RBCs), E. histolytica/E. dispar trophozoites and other intestinal parasites. The specimens were further tested for the presence of E. histolytica/E. dispar DNA by PCR; for stool antigens of E. histolytica by antigen ELISA; and by stool culture for dirrheagenic bacteria. Microscopic observations revealed E. histolytica/E. dispar to be the most common parasite diagnosed (40.7% of the diarrheic patients) followed by Blastocytis hominis (24.7%) and Giardia lamblia (12.7%). However, the PCR based method which amplifies short tandem repeating gene sequence on the tRNA confirmed only 10 cases (16.4%) of E. histolytica/E. dispar. Out of these only two (3.3%) were the pathogenic E. histolytica .The Entamoeba specific stool antigen ELISA detected 30 cases (20%) to be E. histolytica /E. dispar. However, only two of the ELISA positive cases were confirmed by the PCR based method. Of the 150 diarrheal patients, 57(38%) were Shigellae and 1(0.4%) Escherichia coli B. Infection with trophozoites of Entamoeba species showed no clear association with clinical symptoms such as history of bloody-mucoid stools, history of low-grade fever, and microscopic finding of pus cells and RBCs(P>0.05). On the other hand, the isolation of Shigellae in patients with these clinical symptoms was significantly higher (P<0.05). High rate of co-infection in patients with trophozoites of Entamoeba spp. were observed most commonly with Shigella spp. (41.3%), Blastocystis hominis (32.8%), Giardia lamblia (14.8%). Consistent with the previous reports, the present study indicated the rare occurrence of E. histolytica in Ethiopia and the inadequacy of microscopy for diagnosis of amoebiasis. The finding in this study reinforces the suggestion that patients with enteric bacterial pathogens may be wrongly diagnosed as suffering from amoebiasis simply due to the presence of non-pathogenic amoeba trophozoites in their stools. However, the contribution of enteric coccidians, like Cryptosporidium parvum, Isospora belli, and Microsporidia must also be assessed to more correctly establish the etiologyof diarrhea in Ethiopia. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/20307 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Entamoeba Histolytica | en_US |
| dc.subject | E. Dispar | en_US |
| dc.subject | Entreic Bacteria | en_US |
| dc.subject | Co-Infection | en_US |
| dc.subject | PCR | en_US |
| dc.subject | ELISA | en_US |
| dc.title | Entamoeba Species Commonly Diagnosed as Entamoeba Histolytica and Enteric Bacteria in the Pathogenesis of Diarrhea in Addis Ababa. | en_US |
| dc.type | Thesis | en_US |