Investigation of the effect of coffee on body weight, serum glucose, uric acid and lipid profile levels in male albino wistar rats feeding on high-fructose diet
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Date
2018-06
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Addis Ababa Universty
Abstract
Background: High dietary intake of fructose is an important nutritional factor in the development of metabolic syndrome (MetS) and its associated complications. Consumption of coffee, functional foods such as vegetables and fruits are believed to have protective effects against MetS.
Objective: The main objective of the present study was to investigate the effect of coffee on body weight, serum glucose, uric acid and lipid profile levels in male albino Wistar rats feeding on high fructose diet.
Methodology: A post-test experimental study was conducted on a total of 30 (9-10 weeks old) male albino Wistar rats. The rats were divided into 6 groups: group I (normal control)-fed on standard chow and plain tap water only; group II (fructose control)-fed on standard chow and 20% of fructose solution; group III–VI (treatment groups)-fed on standard chow, 20% of fructose solution and treated with 71, 142, 213 and 284mg/kg body weight/day of coffee respectively for six weeks. At the end, diet consumption, body weight, serum glucose, uric acid and lipid profile levels were investigated. Data was entered by Epi-data v. 3.1 and analyzed by one way ANOVA followed by Tukey post hoc multiple comparison tests using SPSS V. 23.00. Statistical significance was considered at p < 0.05.
Results: Body weight, fasting serum glucose and uric acid levels significantly lowered in rats treated with 213 (p = 0.047; 0.049; 0.026) and 284 (p = 0.035; 0.029; 0.010) mg/kg body weight/day of coffee compared to fructose control group. Fasting serum triglycide (TG) and low density lipoprotein (LDL-C) levels showed significant reduction in rats treated with 284mg/kg body weight/day of coffee as compared to fructose control group (p = 0.031; 0.046) respectively. Statistically significant difference was not found in fasting serum levels of TC and HDL-C between coffee treatment groups and fructose control group (p > 0.05). Body weight was positively correlated with serum glucose (r = 0.546, p = 0.002), uric acid ( r = 0.560, p = 0.001), TC (r = 0.435, p = 0.016), LDL-C (r = 0.473, p = 0.008) and TG (r = 0.344, p = 0.063) levels and negatively correlated with HDL-C level (r = - 0.216, p = 0.253).
Conclusion: Treating rats with coffee decreased body weight, fasting serum glucose, uric acid, TC, TG and LDL-C, and increased HDL-C in a dose dependent manner in rats feeding on high fructose diet, suggesting that coffee consumption may be helpful in ameliorating metabolic syndrome.
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Keywords
Body weight, Coffee, Glucose, High-fructose diet, Lipid profiles, Uric acid