Evaluation of total oxidative stress and creatine kinase (bb) level for screening and diagnosis of brain tumor among brain tumor patients attending Tikur Anbessa Specialized Hospital. BY
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Date
2018-10
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Addis Ababa Universty
Abstract
Background: The exact cause of brain tumors is still unknown, however, it is an important cause of morbidity and mortality of both adults and children, often generating severe disabilities and producing high burden in the health care systems. A recently growing advanced laboratory research has shown that oxidative stress can facilitate brain tumor initiation, growth and metastasis by altering tumor cell biology and microenvironment. Scholars are searching for an early biomarker for diagnosis, prognosis and potential target for treatment by looking at tumor metabolism as almost all type of cancer need high energy input and substrate via aberrant metabolism for survival. Therefore, the present study focused on investigating and examining metabolic activity of creatine kinase brain isoenzyme and oxidative stress in brain tumor patients for a potential use as a Biomarker for screening and early diagnosis of brain tumor.
Aim of the study: To evaluate total oxidative stress and Creatine kinase (BB) level for screening and diagnosis of brain tumor among brain tumor patients attending Tikur Anbessa specialized hospital, Addis Ababa, Ethiopia, from April 2018 - October 2018.
Material and methods: Hospital based comparative cross-sectional study was conducted on a total of 90 study participants (50 brain tumor patients and 40 healthy controls). The parameters measured were serum levels of total oxidative stress and creatine kinase BB. Purposive sampling technique was implemented to select study participant in the hospital. This was a collaborative work between Departments of Biochemistry, Neurology and Oncology center.
Results: The present study result demonstrated that total oxidative stress and activity of creatine kinase BB were significantly increased in the serum (10.34±3.23μmoleH2O2eqv./l, 35.12±31.25 μg/L) of brain tumor patients as compared to serum control groups (3.87±2.62 μg/L, 6.9467±2.91μmoleH2O2eqv./l) (p<0.05). Total oxidative stress level of malignant brain tumors (12.76 ±3.38μmoleH2O2eqv./l) were significantly higher than benign tumor types (8.86±2.03μmoleH2O2eqv./l) (p < 0.05) but creatine kinase BB level were not significantly different between benign(38±34.33 μg/L) and malignant(25.98 ±25.98μg/L) types of brain tumor (p > 0.05). Both serum level total oxidative stress (p= 0.001, OR= 1.535, CI=1.247-1.889) and creatine kinase BB (p=0.001) were significantly associated with brain tumor.
Conclusion: Catalytic activity of creatine kinase BB and serum total oxidative stress were significantly increased among brain tumor parents when compared to serum levels of apparently healthy control groups. The serum level of total oxidative stress and creatine kinase BB were not significantly different and correlated among different brain tumor sizes. Creatine kinase BB activity was significantly associated with brain tumor and may be simple non-invasive biomarker for diagnosis of suspected cases of brain tumor. Participants with increased total oxidative stress (brain tumor patients) have significantly high risk of brain tumor development compared to lower serum value (healthy controls).
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Keywords
Brain Tumor, Creatine Kinase Brain Isoenzyme, Oxidative Stress.