The Role of Crude Ethanol Extract of Ginger Rhizome (Zingiber Officinale) on the Liver Biomarkers of CCl4 –Induced Hepatotoxicity in Male Wistar Albino Rats.
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Date
2012-06
Authors
Journal Title
Journal ISSN
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Publisher
Addis Ababa University
Abstract
Introduction: Liver, an imperative organ has a crucial role in the metabolism of xenobiotics that causes it
to succumb to numerous hepatic diseases. Synthetic drugs exploited in the treatment of liver diseases are
incompetent and may sometimes lead to serious side-effects. In this context, herbal therapy has emerged
as a proficient approach with good values in treating hepatic diseases. Ginger have been proven to be
effectual as hepatoprotective agent and it may serve as a vital source of potentially useful new compounds
for the development of effective therapy to combat a variety of liver problems caused by many toxins
including carbon tetrachloride.
Objectives: The present study was designed to explore the role of crude ethanol extract of ginger rhizome
on the biomarkers of CCl
induced hepatotoxicity in male Wistar albino rats.
Methods: Twenty eight rats were used throughout the study. Rats were divided into four groups with
seven in each. Group 1 (normal control) received 1mL/kg of corn oil, group 2 (standards) received
350mg/kg vitamin E, group 3 (treated) received 200mg/kg crud ginger extract, group 4 (negative control)
received 0.5mL/kg CCl
4
4
. Liver toxicity was induced by intraperitoneal administration of CCl
dissolved
in 1mL corn oil at a dose of 0.5mL /Kg body weight.
Administration of crude ethanol ginger extract at a dose of 200 mg/kg body weight was started a week
after CCl
treatment and was performed for five weeks (totally six weeks).
Results: The results revealed that hepatotoxic rats supplemented with 200mg/kg showed a significant
decrease in MDA, ALT and TP with the same p-value (p<0.01) and AST (p<0.001) as compared to
negative control. ALP (p<0.01), DB (p<0.05) and TB (p<0.001) in treated groups compared to the
negative control, but no significant difference was observed in treated, normal and the standards. Serum
TC, LDL-c, and TG were increased significantly (P<0.05) in CCl
4
treated group, while HDL-c level was
decreased significantly compared to the rest of the groups. In ginger treated group, the serum levels of
TC, LDL-c, and TG were significantly (p<0.001) decreased, but HDL-c was significantly (p<0.05) higher
than negative control. Serum levels of TC, LDL-c, and TG in standards were decreased significantly
(P<0.001), but HDL-c increased none significantly compared to negative control. The levels of TC, LDLc,
HDL-c,
and
TG
in
treated
group
were
non-significantly
different
compared
to
the
normal
control
and
the
standards.
Conclusion:
The
study
revealed
that
ginger
rhizome
showed
a
promising
hepatoprotective
effect,
because
it
contains
antioxidants
and potent lipid lowering phytoconstituents. Despite the above, further study is
recommended to investigate the active ingredients of the extract and their exact mechanism of action.
Description
Keywords
Zingiber officinale; hepatoprotective; antioxidant; carbon tetrachloride, MDA, Liver function enzymes, lipid profiles.