ISOLATION, IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY TEST OF ESCHERICHIA COLI O157 AND NON-TYPHOID SALMONELLA SPECIES IN MILK AND FAECES OF LACTATING COWS AND CAMELS IN BORANA PASTORAL AREA, SOUTHERN ETHIOPIA
| dc.contributor.advisor | Dr. Kebede Amenu, Dr. Silvia Alonso | |
| dc.contributor.author | DIRIBA, HUNDUMA | |
| dc.date.accessioned | 2018-09-26T07:54:03Z | |
| dc.date.accessioned | 2023-11-30T12:54:16Z | |
| dc.date.available | 2018-09-26T07:54:03Z | |
| dc.date.available | 2023-11-30T12:54:16Z | |
| dc.date.issued | 2018-06 | |
| dc.description | A thesis submitted to the College of Veterinary Medicine and Agriculture of Addis Ababa University in partial fulfillment of the requirements for the Degree of Master of Veterinary Science in Veterinary Microbiology | en_US |
| dc.description.abstract | A cross-sectional study was conducted to estimate the prevalence and determine antimicrobial susceptibility profile of E. coli O157 and non-typhoid Salmonella species in milk and feces of apparently healthy lactating cows and camels managed under extensive husbandry systems in Borana pastoral areas of southern Ethiopia. A total of 300 samples from cattle and 188 samples from camel were systematically collected and examined to isolate and identify both pathogens following the standard techniques and procedures. The prevalence of E. coli O157was 4.67% in both milk and feces samples in cows and the prevalence of Salmonella was 4% and 8.6% in cattle milk and feces respectively. The prevalence of E. coli O157 in camels’ feces was 3.29%. But no isolate was observed in camel milk samples. On the other hand, 1.06% and 2.13% of milk and feces from camels were positive for Salmonella, respectively. All of the E. coli O157 and Salmonella isolates from both animal species showed high susceptibility to Nalidixic acid (100%), Gentamicin (100%) Ciprofloxacin (100%) and Chloramphenicol (90.9%), whereas, 92.86% (13/14) of E. coli O157 and 15.79% (3/19) of Salmonella isolates from cattle showed resistance to two or more antimicrobial agents. However, none of E. coli O157 isolates from camels found resistant to two or more antimicrobial agents while 66.66% (2/3) of Salmonella isolates were multiple antimicrobial resistant. The highest level of resistance was observed for Ampicillin (100%) in both animal species for all isolated pathogens followed by Tetracycline (58.8%) for E. coli O157 in cattle. In the current study, considerable proportions of milk and fecal samples harbored drug resistant E. coli O157 and Salmonella species which have a significant public health risk. Therefore, relevant intervention programs and awareness creation on best practice of milk handling are essential to minimize the risk associated with consumption of contaminated milk. Education on the control and surveillance program of antimicrobial usage in animals and animal products are recommended to ensure consumer safety. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/12255 | |
| dc.language.iso | en | en_US |
| dc.subject | Camel | en_US |
| dc.subject | Escherichia Coli O157 | en_US |
| dc.subject | Salmonella | en_US |
| dc.title | ISOLATION, IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY TEST OF ESCHERICHIA COLI O157 AND NON-TYPHOID SALMONELLA SPECIES IN MILK AND FAECES OF LACTATING COWS AND CAMELS IN BORANA PASTORAL AREA, SOUTHERN ETHIOPIA | en_US |
| dc.type | Thesis | en_US |