In-vivo Anti-inflammatory Activity of the Leaf Extracts and Fractions of Ocimum Suave in Mice Model
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Date
2011-10
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Addis Abeba University
Abstract
Ocimum suave has been used in Ethiopian traditional medicine to relieve pain, fever,
inflammation and possibly other diseases conditions. Although there are some reports on
analgesic, antipyretic, antiulcer, antimicrobial and wound healing effects, no study has been
made regarding their possible anti-inflammatory effect. The objective of the present study
was, therefore, to investigate ethanol and aqueous extracts and fractions (butanol and water
residue) of the aqueous extract of the plant material. The ethanol and aqueous extract were
screened for their anti-inflammatory activities on carrageenan-induced mouse paw edema at
three dose levels 400, 600 and 800mg/Kg. The butanol and water fractions of the aqueous
extract were also investigated for their anti-inflammatory activities on carrageenan, histamine
and serotonin-induced mouse paw edema at three dose levels 50, 100, 200mg/Kg. Normal
saline and aspirin (200mg/Kg) were employed as negative and positive control groups,
respectively. Both ethanol and aqueous extracts significantly decreased carrageenan-induced
inflammation at all doses used. Greater paw edema inhibition was, however, observed by the
aqueous extract than ethanol one at 600 and 800mg/Kg dose levels. Similarly, both butanol and
water fractions also showed significant reduction of inflammation induced by carrageenan,
histamine and serotonin in mice paw. Highest inhibition, however, was exhibited by the
aqueous fraction at 100mg/Kg and 200mg/Kg dose levels. From the present findings, it can be
concluded that the extracts and fractions of the plant material have got anti-inflammatory
properties. Thus the results support the traditional use of this plant in inflammatory conditions.
Further investigation, however, should be pursued to identify the exact mechanism (s), isolate
the bioactive principle (s) responsible for the anti-inflammatory action and others.
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Keywords
Inflammation,Diseases,Anti-inflammatory Drugs