Phylogenetic Analysis and Diversity of1 Envelope V3 Sequences from Seroconverters of the two Ethiopian Cohorts: Akaki & Wonji
| dc.contributor.advisor | Bekele Endashaw (Professor) | |
| dc.contributor.author | Hagos Measho | |
| dc.date.accessioned | 2018-07-03T13:17:49Z | |
| dc.date.accessioned | 2023-11-08T16:32:46Z | |
| dc.date.available | 2018-07-03T13:17:49Z | |
| dc.date.available | 2023-11-08T16:32:46Z | |
| dc.date.issued | 2001-06 | |
| dc.description.abstract | HIV-I is characterized by its genetic variability. Based on nucleotide sequence relatedness. the HIV -I virus variants are grouped into three groups: M (major). 0 (outlier). and N (non-M or non-OJ. Group M viruses are further divided into 9 genetic subtypes or clades (A-D. F-H. J. & K). The Ethiopian HIV-l/AIDS epidemic is reported to be dominated by HIV-I subtype C. which recently is reported to have a genetic subcluster designated as C'. In an effort to determine the diversity of gp120 V3 sequences and the frequency of subtype C and C' viruses among HIV -I isolates from recently infected individuals. 19 Ethiopian factory workers who seroconverted for HIV -I antibodies during the year 1997-2000 were included in the study. HIV-I RNA was isolated from plasma samples of study subjects. the RNA was reverse transcribed into cDNA and the envelope gpl20 V3 region was amplified. Phylogenetic analysis of the env V3 sequences showed all the 19 subjects to be infected by subtype C. II out of the 15 sequences were found to be subtype C' and 4 SUbtype C. Based on this observation we speculate that subtype C' viruses are spreading and might have a selective advantage for transmission. Amino acid sequence comparison of the two groups of viruses showed significant amino acid sequence differences. There was a 17% synonymous and 15% non synonymous distance among all the seroconverter sequences and a 24% synonymous and 17% nonsynonymous distance between the C and C' group of sequences. The synonymous distance among subtype C'(9%) is much less compared to the distance among the SUbtype C sequences (16%). | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/6024 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Biology | en_US |
| dc.title | Phylogenetic Analysis and Diversity of1 Envelope V3 Sequences from Seroconverters of the two Ethiopian Cohorts: Akaki & Wonji | en_US |
| dc.type | Thesis | en_US |