Morphological and Biocuemical Diversity of Grassplj/L (Lathylws Sativus L) in Etiilol'la
No Thumbnail Available
Date
1999-05
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
Fifty grasspea populations that were selected based on administrative regions and different
altitude classes were used in tins study. A total of 1199 entries were evaluated for 14 qualitative
and quantitative morphological traits. Oxalyl diamino propanoic acid (ODAP) variation
was determined for 150 entries by taking three entries fi'Om each population. Protein content
was detenrriued for the 50 grasspea populations following a standard procedure. TIle phenotypic
variations in three enzyme systems: esterase(EST EC 3.1.1.), acid phosphatase (ACP:EC
3.1.3.2), and aspmiate amino tranferase(AAT:EC 2.6.1.1) was investigated using horizontal
starch gel electrophoresis for 10 populations (25 entries per population) selected from the
cluster analysis result based on the morphological data.
Mean, coefficient of variations, heritability, genetic advance, cOlTeiation coefficients, cluster
analysis, factor analysis and Shannon's diversity index estimate were used to estimate the
morphological variation.
ANOVA (analysis of variance) result indicated that there was significant variation for the
different traits witlrin a population and between populations. The coefficient of variation
was large for most traits in Gonder indicating more diversity in this region. Tins was fmiher
asceliained by Shannon's diversity index where the mean diversity (H' ) was the highest
in this region (0.65) followed by Tigray region (H' = 0.64). H at population level ranged
form 0 (monomorphic) to 0.9 (polymorphic) for some traits. The over all mean diversity
for Ethiopia was estimated to be H' =0.61.
Chi-square analysis also showed that there was highly significant variation for each traits
(seed size, flower colour, leaflet size and pattem of testa). Some variation in altitude groups
was also noted though it was not significant. Analysis of variance for mean ODAP content
and protein content showed non- significant differences between populations. However,
ODAP analysis from single plants showed significant variation both with in a population
and between populations. Fortunately, four individual plants with low ODAP contents
ranging from 0.14% to 0.18% (range with in safe level) were obtained from populations
collected from different regions indicating the diversity and variation of this trait with in
population and between regions. These low ODAP lines are obviously considered important
for further breeding program.
Phenotypic polymOlphism was obselved for the three enzymes. High phenotypic polymOlphism
was observed for esterase. There was negative bnt non significant (t= -0.25) association
between morphological diversity index and mean expected hetrozygosity base on isozyme
data.
Key words:Lthyrus sativa, grasspea, lathyrism, diversity, morphological characters,
ODAP, protein, isozyme, conselvation.
Description
Keywords
:Lthyrus sativa, grasspea, lathyrism, diversity, morphological characters, ODAP