In Vitro Regeneration of Niger (Guizotia Abyssinica Cass.) Using Cotyledon and Hypocotyl Explants
No Thumbnail Available
Date
2007-08
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
Callus induction and shoot formation from hypocotyls and cotyledons of
Guizotia abyssinica Cass. has been achieved in this experiment. The
frequency of callus induction was influenced by the concentrations and types
of growth regulators. Six to eight-day-old hypocotyl segments and
cotyledons were cultured on MS medium containing different concentrations
of NAA, IAA and BAP. Among the various concentrations tested, 0.5 mg/l
NAA in combination with 1 mg/l BAP was found to be the best for
maximum callus induction of hypocotyl explants. Furthermore, 2 mg/l IAA
in combination with 1 mg/l BAP was the best for callus induction of
cotyledonary explants. Highest percentage of shoot formation (60%) was
obtained when cotyledons were cultured on medium supplemented with 3.0
mg/l IAA in combination with 1.0 mg/ l BAP. Maximum number of shoots
per explant (20.3) was obtained from medium containing 0.1 mg/l NAA in
combination with 1 mg/l BAP. The types of explant, growth regulator
combinations and genotypes were showed significant effect on shoot
regeneration. The elongated shoots were successfully rooted on media
supplemented with IBA at a concentration of 0.5 mg/l. The shoots were
established in soil where 65% of them survived. Morphologically aberrant
plants were not observed. As plant regeneration protocol is a prerequisite for
genetic transformations, this protocol can be used for such purposes and
development of new varieties with desired traits by in vitro selection
Description
Keywords
Biology