Isolation and Molecular Characterization of Marek's Disease Virus isolates Circulating in Central Tthiopian Chickens
| dc.contributor.advisor | Ayelet, Gelagay (PhD) | |
| dc.contributor.author | Demeke, Berhan | |
| dc.date.accessioned | 2018-06-27T12:32:56Z | |
| dc.date.accessioned | 2023-11-30T12:54:16Z | |
| dc.date.available | 2018-06-27T12:32:56Z | |
| dc.date.available | 2023-11-30T12:54:16Z | |
| dc.date.issued | 2014-06 | |
| dc.description.abstract | Marek´s Disease (MD) is a lymphoproliferative and neuropathic disease of domestic chickens, and less commonly, turkeys and quails, caused by a highly contagious, cell-associated, oncogenic herpesvirus. In Ethiopia MD is believed to be introduced with the exotic and cross-breeds, and has been reported to be a potential threat to both local and exotic breeds in backyard and commercial farming systems. Therefore, the objective of this study was to isolate, identify and molecular characterization of MD virus circulating in the chicken population of the country. Clinical and post-mortem examination was carefully conducted from September, 2013 to December, 2013 in central Ethiopia on clinically diseased chickens suspected of MD virus infection. Representative post-mortem feather follicle and spleen samples were collected under sterile condition and virus isolation was performed using chicken fibroblast cell culture. Cell cultures inoculated with suspension of pathological samples developed characteristic MD virus cytopathic effect of rounding of the cells and small plaques. Further confirmation of the virus was conducted by conventional polymerase chain reaction using MD virus specific primers targeting to amplify the ICP4 gene on twelve samples and revealed the expected band size of 318 base pair of the 11 samples. Eleven positive PCR products were further subjected for sequencing using the amplification primers and the sequence analysis result using the available bioinformatics software’s confirmed that the chickens were infected with MD virus. Phylogenetic tree construction was performed to see the genetic relatedness of the present virus isolates with the reference MD virus strains retrieved from the Genbank and revealed that the virus infected the Ethiopian chickens were clustered under Gallid Herpes virus type 2. To our knowledge, the present study is the first report conducted on virus isolation, molecular characterization and sequencing of MD virus isolates in Ethiopia. xiii Further research work on isolation and molecular characterization of MD virus is recommended by observing chickens at all age groups showing clinical signs of the disease reared under backyard and commercial farming system. Finally, production of an effective MD vaccine in the country which could help in protecting the chickens from the circulating virus type is highly recommended. Key words: Marek’s disease, Marek’s disease virus, molecular characterization, polymerse chain reaction, virus isolation, sequencing | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/4222 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababauniversity | en_US |
| dc.subject | Marek’s diseas | en_US |
| dc.subject | Marek’s disease virus | en_US |
| dc.subject | molecular characterization | en_US |
| dc.subject | polymerse chain reaction | en_US |
| dc.subject | virus isolation | en_US |
| dc.subject | sequencin | en_US |
| dc.title | Isolation and Molecular Characterization of Marek's Disease Virus isolates Circulating in Central Tthiopian Chickens | en_US |
| dc.type | Thesis | en_US |