Antimicrobial Resistance of Staphylococcus aureus and Pseudomonas aeruginosa in Fish, and Knowledge, Attitude and Hygienic Practices of Fish Handlers in Bishoftu, Ethiopia
| dc.contributor.advisor | Takele Beyene | |
| dc.contributor.advisor | Dr. Liyuwork Tesfaw | |
| dc.contributor.author | Kebadu Endeg | |
| dc.date.accessioned | 2024-11-04T11:34:40Z | |
| dc.date.available | 2024-11-04T11:34:40Z | |
| dc.date.issued | 2024 | |
| dc.description | MSc Thesis | |
| dc.description.abstract | Fish, a protein-rich food, can sometimes be contaminated with bacterial pathogens like S. aureus and P. aeruginosa, known for their resistance to antimicrobials. A cross-sectional study was conducted in Bishoftu, Ethiopia, from November 2023 to May 2024. The purpose of the study was to determine the antimicrobial resistance (AMR) pattern of these pathogens, as well as assess the knowledge, attitude towards antimicrobial use (AMU) and AMR, and hygienic practices of fish handlers. The study used a purposive sampling strategy. S. aureus and P. aeruginosa were confirmed using polymerase chain reaction and their susceptibility to antibiotics was tested using the Kirby Bauer disk diffusion method. The data was analyzed using STATA version 14, using descriptive statistics, Chi-squared, likelihood ratio, and binary logistic regression. The results of the study found that 8.6% (9) of 105 samples tested positive for S. aureus and 7.6% (8) for P. aeruginosa. S. aureus isolates were 100% resistant to cefuroxime, 44.4% (4/9) to cloxacillin and penicillin G, and 33.3% (3/9) to tetracycline. P. aeruginosa isolates were 100% resistant to cefuroxime, doxycycline, erythromycin, florfenicol, kanamycin, nalidixic acid, trimethoprim/sulfamethoxazole and tetracycline. Multidrug resistance was observed in 33.3% (3/9) of S. aureus isolates and 100% of P. aeruginosa isolates. None of the S. aureus isolates were positive for mecA gene. The survey revealed that 79% (42/53) and 60% (32/53) of respondents had insufficient knowledge and desirable attitudes about AMU and AMR, respectively and 41% (22/53) had poor hygienic practices. A significant positive correlation was found between respondent’s educational level and knowledge and attitude scores. Gender also played a role in attitude and hygienic practices. The study provides insights into S. aureus and P. aeruginosa resistance in fish production and establishes a baseline understanding of knowledge, attitudes, and hygiene practices among fish handlers. Further research should focus on detecting AMR genes in aquaculture. Additionally, enhancing knowledge about AMU and AMR in aquaculture for fish handlers is of utmost importance. | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/3535 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Ababa University | |
| dc.subject | antimicrobial resistance | |
| dc.subject | attitude | |
| dc.subject | fish handlers | |
| dc.subject | hygienic practices | |
| dc.subject | knowledge | |
| dc.subject | P. aeruginosa | |
| dc.subject | S. aureus. | |
| dc.title | Antimicrobial Resistance of Staphylococcus aureus and Pseudomonas aeruginosa in Fish, and Knowledge, Attitude and Hygienic Practices of Fish Handlers in Bishoftu, Ethiopia | |
| dc.type | Thesis |