Morphological and Molecular Characterizations of wilt disease of Ginger (Zingiber officinale Roscoe) Caused by Fusarium sp
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Date
2015
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Addis Ababa University
Abstract
Ginger rhizome, pseudostem, leaf and soil samples were collected from ginger growing
areas of southwestern Ethiopia. Fungal pathogens were isolated from ginger parts
showing vascular browning symptoms and soil following standard methods. Isolation
revealed fungal isolates belonging to four genera: Fusarium, Penicillium, Aspergillus
andTrichoderma. Out of 24 fungal isolates 14 isolates were Fusarium species. In
vitropathogenicity test resulted in pathogenicity index (PI) values ranging from 10.96%
+1.55(AAUFG13) to 45.35% +11.57 (AAUFG6). Cultures of Fusarium species on
PDAmedium showed white, creamy white, dull pink and pink coloration. They
imparted dullwhite, orange, light reddish purple, intense reddish purple and dark
reddish purple pigmentations on the riverse side. Canoe shaped macroconidia, ovoid microconidia and short phialides were pertinent to all of the Fusarium isolates. Based on their cultural and morphological characters the isolates were identified as Fusarium oxysporum. The ability of the isolates to induce rhizome rot confirmed the forma specials rank of the isolates as
F. oxysporum f. sp. zingiberi (Foz). PCR- RFLP profile of the ITS1 rDNA region
indicated genetic variation between the Fusarium isolates. In silco RFLP digestion of
ITS1 rDNA sequences of F. oxysporum retrieved from the fungal ITSoneDB was
performed using online restriction mapper software and restriction enzymes used in the
actual experiment. The result of the in silco restriction digestion simulation was similar
with the actual PCR_RFLP study supporting that the isolates are Fusarium oxysporum.
Key words: Fusarium wilt; ITS1;Pathogenicity; PCR-RFLP; Rizhome rot
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Keywords
: Fusarium wilt; ITS1;Pathogenicity; PCR-RFLP; Rizhome rot