Drug sensitivity patterns of Mycobacterium tuberculosis complex species isolated from tuberculosis lymphadenitis patients in north-western Ethiopia
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Date
2017-04
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Addis Ababa University
Abstract
Drug resistance threatens the National Tuberculosis Control Program in several countries of the world. The drug sensitivity patterns, especially among tuberculosis lymphadenitis (TBLN) patients, are not fully understood in northwestern Ethiopia. This study aimed to identify drug sensitivity patterns of Mycobacterium tuberculosis complex (MTBC) strains isolated from TB lymphadenitis (TBLN) patients and association of strain types in northwestern Ethiopia. Study design was convenient sampling. Drug sensitivity testing (DST) against first line drugs, including isoniazid (INH), streptomycin (SM), rifampicin (RMP) and ethambutol (EMB), was performed using Mycobacterium Growth Indicator Tube (BACTEC MGIT 960) system and GenoType MTBDRplus assay. The study showed TBLN patients to harbor drug-resistant and MDR-TB. Among 82 MTBC isolates, 71 (86.5%) were sensitive to all first-line drugs and only 1 (1.2%) was multi-drug resistant (MDR).Any resistance to INH, RMP, SM and EMB was 3(3.65%), 2 (2.43%), 8 (9.75%) and 1(1. 20%), respectively. This study showed that TBLN patients harbored drug-resistant TB and MDR-TB in north-western. Euro-American was the dominant lineage identified from 52.4% (43/82) of the isolates and 2(2.4%) of the strains were M. Bovis. Although the prevalence of MDR-TB low, detection high resistance of first line drug, Ionized, is an indication of its possible spread in the study area. It requires to strengthen national control Program in its effort to prevent and control TB and a close drug resistance monitoring to keep it low and ultimately eliminate it. Keywords: TB lymphadenitis, drug resistance, mycobacterium TB complex strains, BACTEC MGIT 960, GenoType MTBDRplus assay.
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TB lymphadenitis, drug resistance, mycobacterium TB complex strains, BACTEC MGIT 960, GenoType MTBDRplus assay