Extraction and Purification of Betanin from Red Beetroots and Synthesis of Co(Ii), Cu(Ii) and Zn(Ii) Based Betanin Metal Complexes
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Date
2020-02-02
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Addis Ababa University
Abstract
For this study, red beetroots were used as a source of betanin. Betanin extraction process was carried out by using methanol as a solvent. Column chromatography method was used to purify the extracted betanin and to isolate the purified betanin by slightly increasing the polarity of the solvent. The absorption spectra of the extracted and purified sample was efficiently monitored and recorded in the UV-Vis region (200–800 nm) by using Perkin Elmer, Lambda 950 UV/Vis/NIR scanning spectrometer with methanol reference. As observed from the experimental result, the maximum absorption spectra of pure betanin is around λmax 540 nm is comparable result with absorption spectra data of pure betanin in different literatures. Ferric reducing power method was applied with slight modification to study the reducing capacity of betanin. Reducing power assay method to evaluate antioxidant ability was based on the reduction of Fe3+ to Fe2+ in which the yellow color of the test solution changes to various shades blue, which indicates the reducing capacity of the sample. Purified betanin were subjected to complexation with Co2+, Cu2+ and Zn2+. As observed from the experimental results, maximum absorption spectra of newly synthesized betanin-metal complexes showed blue shifting absorption spectra compared with absorption spectra of pure betanin. Finally, X-ray diffraction technique was used to draw conclusion about the structure and phase purity of the newly synthesized metal complexes. X-ray diffraction spectra results showed that the constituents of the resulting betanin-metal complex solids are arranged with no particular order indicating they are amorphous solids.
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Beetroot, Betanin, Stability, Super food, Medicinal plant, Oxygen, Temperature, Light and Antioxidant