Prevalence of Escherichia Coli O157:H7 and Antimicrobial Resistance Profile in Dairy Farms and in Contact Humans in Bishoftu and Modjo Towns
| dc.contributor.advisor | Teshale Sori (DVM, MSc, PhD, Professor) | |
| dc.contributor.advisor | Addisu Demeke (DVM, MSc, PhD, Asso. Prof.) | |
| dc.contributor.author | Taye Dinku | |
| dc.date.accessioned | 2025-12-01T08:51:32Z | |
| dc.date.available | 2025-12-01T08:51:32Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | E. coli O157:H7 poses a serious challenge to public health worldwide with ongoing acquisition of resistant to different antimicrobials. The cross-sectional study with stratified and simple random sampling was conducted to isolate and assess antimicrobial resistance profile of E. coli O157:H7 from dairy cattle, milk, in contact humans, soil and different water sources used in dairy farms as well as assess the associated risk factors of E. coli O157:H7 infection in dairy farms and in contact humans in Bishoftu and Modjo towns. Three hundred eighty two (382) containing cows’ feces, milk, stool and environmental samples were collected and processed using bacteriological isolation, biochemical identification and confirmed by serological assay. Descriptive analyses were used to compute the prevalence of E. coli O157:H7 and the association between the outcomes and independent variables was analyzed using chi-square test at p value <0.05 and 95% confidence interval. Overall prevalence of E. coli O157:H7 was found to be 3.9%. The occurrence in soil and floor swab, fecal, stool 6.7%, 5.7%, 4.6%, respectively while it was 3.3% in milk, water and udder swab samples. There was no occurrence of E. coli O157:H7 detected on equipment and hand swabs. The occurrence of E. coli O157:H7 in fecal sample was significantly associated with floor type, cleaning frequency of the pen, pen cleanness and manure management sites. The prevalence in milk samples showed a statistically significant association (p<0.05) with factors like floor type, ways of udder cleaning, hand washing practice and manure management sites. Occurrence in soil samples was significantly associated (p<0.05) with floor type and manure disposal site while only manure management sites had statistically significant association (p<0.05) with occurrence in water samples. Risk factors such as age, drinking of raw milk, history of diarrhea and hand washing practice were found to be statistically significantly associated with E. coli O157:H7 occurrence in human. antimicrobial sensitivity test to selected drugs revealed that all of the isolates (100%) were susceptible to nalidixic acid while all of the isolates (100%) were resistance to ceftazidime with multidrug resistance observed in 86.7% of the isolates. Therefore, there should be strict dairy farm hygienic practices such as manure management and rational uses of drug were recommended. | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/7558 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Abeba University | |
| dc.subject | Antimicrobial resistance | |
| dc.subject | Children | |
| dc.subject | Dairy farm | |
| dc.subject | Escherichia coli O157:H7 | |
| dc.title | Prevalence of Escherichia Coli O157:H7 and Antimicrobial Resistance Profile in Dairy Farms and in Contact Humans in Bishoftu and Modjo Towns | |
| dc.type | Thesis |