Amperometric Determination of Potassium and of Urease Activity in Plant Materials
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Date
1997-06
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Addis Ababa Universty
Abstract
Determination of potassium and urease activity of locally found plant seeds have been made.
The membrane-stabilized water-nitrobenzene interface was used as an al11pcrometric sensor.
The electroanalytical teclmiques employed were cyclic voItammetry, ac-voltanullelly and
clu·onoamperomelly.
The sensor was calibrated by the standard addition method. The detection of 0.025 mmol 1"
of potassium ion as well as ammonium ion was possible. Since the sodium ion content of the
seed powders investigated was extremely low, no interference of sodium ion was observed.
Several plant seeds have been screened for urease activity. The transfer of anullonium ion
produced by enzymatic hydrolysis of urea was followed by the sensor.
Three new sources of enzyme urease have been found, namely, Kil (lagena/'ia siceraria),
Berbera (iv!illitiafemiginea), and Gibto (lupinus a/bus).
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Keywords
Potassium and Urease activity