Reference value determination of CD4+ T-Lymphocyte in HIV Sero- Negative Ethiopian pregnant women
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Date
2009-07
Authors
Journal Title
Journal ISSN
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Publisher
Addis Abeba University
Abstract
In Ethiopia, reference intervals in clinical laboratories are commonly based on results obtained
for western populations or from test kit manufacturers. The present cross sectional study aimed
to establish reference interval for CD4
+
T lymphocyte counts, in HIV sero-negative pregnant
women attending antenatal care clinic at Yekatit 12 hospital.
Flow cytometric analysis was performed to determine CD4
+
and total T lymphocyte counts.
Accordingly gestational ages between 4 and 38weeks (n=120) were used. Reference values
obtained were 310-1185 cells/µl for Absolute CD4
+
T cells count, 17.9-54.0% for CD4
T cells
percentage, 690-2540 cells/µl for absolute CD3
+
T cells count, 55-84% for CD3
T cells
percentage, 952-2985 cells/ µl for absolute CD45
+
lymphocyte count.
The data revealed that CD4
absolute CD4
+
+
T cell percentage was stable throughout the pregnancy period while
T cell count varies between trimesters indicating that CD4
+
T cell percentage
could be more reliable in the management of HIV infected pregnant women than the absolute
value. In conclusion, T lymphocyte populations in pregnant women were different from non
pregnant women. The clinical implication of these differences is the initiation of antiretroviral
therapy (ART) in HIV infected pregnant women.
Description
Keywords
CD4+ ,T-Lymphocyte ,HIV, Sero- Negative ,Ethiopian, pregnant women.