Detection and Identification of Yam Mosaic and Yam Mild Mosaic Potyviruses In Major Yam (Dioscorea Spp.) Growing Areas of South-Western Ethiopia
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Date
2018-02-02
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Addis Ababa University
Abstract
Yam is the common name for some plant species in the genus Dioscorea (family
Dioscoreaceae) that form edible tuber. Yams are herbaceous or dioecious annual or
perennial vines cultivated for their edible starchy tuber in Africa, Asia, Latin America
and Caribbean. Yam production is negatively affected by pests and diseases in the
world. Among these, virus diseases are the most important concerns of yam production
as they strictly restrict the exchange of germplasms for various purposes in breeding
programs. Among viruses reported to be infectious of yam crop in the world the genus
Potyvirus is the largest group of viruses in Potyvirideae family and is the most
widespread pathogen of yam virus diseases in the world. Yam mosaic virus (YMV) and
Yam mild mosaic virus (YMMV) are the two commonly reported Potyviruses to be
widespread across yam growing areas of tropics and subtropics. This study was
therefore designed to detect Yam mosaic and Yam mild mosaic Potyviruses in major
yam growing areas of South-Western part of Ethiopia. ACP-ELISA, DAS-ELISA and
RT-PCR were used to test a total of 336 yam leaf samples collected from Jimma
Agricultural Research Center, Bako Agricultural Research Center and Hawassa
Agricultural Research Center for the two common Potyviruses (YMMV and YMV).
Among 336 total samples tested in this study 40 (12%) and 32 (9.5%) of them were
found positives for potyvirus in ELISA and RT-PCR tests respectively. YMV was
found in 30 (75%) and 38 (95%) of samples confirmed positive for potyvirus via DASELISA
and RT-PCR respectively, whereas YMMV is detected in none of the samples.
This study therefore provides valuable information about YMV, to be the important
Potyvirus attacking yams in Ethiopia, and is required to devise effective methods for the
control of YMV disease on yams.
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Keywords
Yam, Potyvirus, Yam Mosaic Virus (YMV), Yam Mild Mosaic Virus (YMMV), Reverse Transcription PCR (RT-PCR) and ELISA