Isolation, Identification of Fusarium Xylarioides (Gibberella Xylarioides) from Southern Ethiopia and its Response to Fungal Biocontrol Agents

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Addis Ababa Universty


Five isolates of Fusarium xylarioides form five coffee growing Weredas in southwestern Ethiopia were isolated from Arabica coffee trees showing sever symptoms of vascular wilt. These strains were characterized based on vegetative and spore morphology and vegetative growth on different growth media, temperature and pH ranges. These strains produced both microconidia and macroconidia on Potato Dextrose Agar (PDA) and Synthetic low-nutrient Agar (SNA). Microconidia were unicellular, allantoids and had 0-1 septate. Macroconidia were strongly curved with marked foot cells and had 1-4 septate. Pathogenicity test was performed for the subsets of isolates by inoculating 20 ml, 40 ml, and 60 ml of spore suspensions. Symptoms developed on the 30th day of inoculation concentration wisely. All seedlings died on the 90 day of inoculation. The controls were drenched with equivalent amount of distilled water and no symptoms developed until the completion of the study. In vitro evaluation of antagonistic test of dual culture of the test isolates with two Trichoderma species showed that the radial growth inhibition of the pathogens. The cell free culture filtrates of different concentration also showed the radial growth inhibition of the test pathogens. Key words: Fusarium xylarioides, Trichodrma harzianum, Trichoderma viride, Biological control.



Fusarium xylarioides, Trichodrma harzianum, Trichoderma viride, Biological control