Wound Healing and Antibacterial Activities of Solvent Fractions of 80% Methanol Leaf Extract of Brucea antidysenterica J .F. Mill (Simaroubaceae)

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Addis Abeba University


Brucea antidysenterica is a medicinal plant used for different aliments including dysentery and non-healing wounds. Wound healing, antibacterial, antiinflammatory and antioxidant activities of the crude leaf extracts were reported to be promising. The aim of this study was to examine wound healing and antibacterial activity of solvent fractions of 80% methanol leaf extract of B. antidysenterica. The crude leaf extract was fractionated with water, n-butanol and chloroform. Solvent fractions were formulated as 2% and 4% ointment bases. Excision and incision models were used to determine wound healing activity. Wound contraction rate, period of epithelialization and breaking strength determined. In vitro antibacterial activities of solvent fractions were tested. 2% and 4% aqueous fractions (AF) showed wound healing activity though n-butanol (BF) and chloroform fractions (CF) appeared to have delayed wound healing or exacerbating effect. At the end of treatment period, 2% and 4% AF significantly increased wound contraction (p<0.001) compared with the negative control. 4% AF significantly shortened (p<0.05) Period of epithelialization. 2% and 4% AF significantly increased tensile strength of wounded skin compared with untreated (P< 0.001). BF had the highest antibacterial activity as shown by inhibiting growth of four bacterial strains. S. aurues was the most susceptible species for BF with MIC of 75mg/ml. The AF of 80% methanol leaf extract of B. antidysenterica possesses wound healing activity. AF, BF and CF evidenced antibacterial activity against selected strains.



Brucea antidysenterica, wound healing activity, Agar well diffusion.