Genetic Diversity Study of the Brachiaria Brizantha (A. Rich.) Stapf Collected From Ethiopia Using Inter Simple Sequence Repeat (Issr) Markers
| dc.contributor.advisor | Tesfaye, Kassahun (PhD) | |
| dc.contributor.advisor | Haileselassie, Teklehaimanot (PhD) | |
| dc.contributor.author | Meheret, Fentahun | |
| dc.date.accessioned | 2020-10-20T11:04:46Z | |
| dc.date.accessioned | 2023-11-18T09:50:14Z | |
| dc.date.available | 2020-10-20T11:04:46Z | |
| dc.date.available | 2023-11-18T09:50:14Z | |
| dc.date.issued | 2014-02-02 | |
| dc.description.abstract | Brachiaria brizantha is a C4 grass commonly used as forage in the tropics. Leaf samples from seven populations originating from Welega, Jimma, Omo, Gondar, Gojam, Borena and Ilu Ababora were collected from Zeway Regeneration and Conservation Site of ILRI, Ethiopia. Genomic DNA was extracted from 79 accessions each from three individuals using CTAB extraction method. A diluted genomic DNA was subjected to PCR amplification. From the ISSR primers tested, only six amplified 80 scorable bands from the 79 accessions used. 96.25% Percentage of polymorphic loci (PPL), gene diversity (h) = 0.366 and Shannon information’s index (I) = 0.539 were detected at species level. The number of bands identified by each primer ranged from 8 to 19, with an average of 13.3. The highest genetic diversity was generated from accessions collected from Ilu Ababora (PPL= 58.75 %, h=0.210 and I =0.315) while the least was from those collected from Gondar region (PPL=28.75%, h=0.124 and I =0.183). Jaccard’s similarity coefficients ranged from 0.302 to 0.431 and analysis of molecular variance indicated the presence of higher proportion of variation within population (64.66%) than between populations (35.34 %). Cluster analysis using the un-weighted paired group method with arithmetic average (UPGMA) and Jaccard’s similarity coefficient (0.38) clustered the accessions into three major (I, II and III) clusters in their respective regional collection. Principal Component Analysis (PCO) showed accessions in populations formed their own cluster. Thus ISSR markers detected a range of genetic diversity from Brachiaria brizantha germplasm collections from Ethiopia. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/22870 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Forage | en_US |
| dc.subject | Genetic Variability | en_US |
| dc.subject | Molecular Markers | en_US |
| dc.title | Genetic Diversity Study of the Brachiaria Brizantha (A. Rich.) Stapf Collected From Ethiopia Using Inter Simple Sequence Repeat (Issr) Markers | en_US |
| dc.type | Thesis | en_US |