Escherichia Coli0157:H7: Prevalence and Sources of Contamination of Cattle Meat at Municipal Abattoir and Butcheries as Well as its Public Health Importance in Debre Brerhan, Ethiopia
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Date
2014-06
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AAU
Abstract
The study was carried out from November 20 13 to April 2014 with the objecti ves of determining
the prevalence and source of contamination of E. coli 0157: H7 from municipal abattoir and
butcher houses, to assess the public health importance of this organism and to lest the drug
sensitivity pattern of the isolates in Oebre-Berhan. A total of 1,132 samples from abattoir and 40
samples from butcher hOllses were examined. Samples from abattoir included hide swabs,
internal and external carcass swabs. fecal sample, intestinal mucosal swabs and environmental
samples (carcass in contacts, i.c. knife, workers hand, and clothing). Likewise, the samples from
butcher houses were swab from carcass, butcher man's hand, cutting board and knife. In
addition, stool samples from suspect individuals visiting Debre-Berhan referral hospital were
collected. The samples were transportcd in Buffered peptone waler to laboratory of the
department of Biological science, Oebre Berhan University in ice box and scrutinized according
to international standards. From 220 animals slaughtered, a total of 1100 sampl es were taken and
only 6 (0.54%) were found to be positive of £. coli 0157:117. E. coli 0157:H7 was isolated from
2 (0.91 %) of hide swab samples, 3 (1.36%) of feces samples and I (0.45%) of intestinal mucosal
swab. There were no isolate found from 32 environmental sampl es, 20 stool samples and 40
swab samples from butcher shops. Prevalence of E. coli 0157:H7 was significantly associated
with the cleanness of the hide (P=0.036). All E. coli 0157:H7 isolates were then checked for
their susceptibility pattern against ten selected antibiotics. The isolates were completely suscepti ble to 7 antibiotics tested, namely Tetracycline (TE, 30 Ilg), Chloramphenicol (C, 30Ilg),
Sulfamethoxazole-Trimitoprim (SXT, 251lg), Ciprofloxacine (eIP, 5Ilg), Nalidixic acid (NA,
30llg), Kenamycine (K . 30llg) and Sterptomycine (S, JOllg) but they were resistant to
Amoxicillin (AML, 25 Ilg). Though, the prevalence of E. coli 0157:1-17 in present study is low,
the impact of the pathogen should not be underestimated.
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Keywords
Antibiotic, Buffered peptone. Debre Berhan, E. coli 0157: H7, Swab Samples