ISOLATION, IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY OF PASTEURELLA MULTOCIDA FROM CATTLE WITH HEMORRHAGIC SEPTICEMIA IN ASSOSA AND BAMBASI DISTRICTS, BENISHANGUL GUMUZ REGIONAL STATE, ETHIOPIA
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Date
2017-06
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Abstract
A cross sectional study was conducted from November 2016 to May 2017 in Bambasi
and Assosa districts in Benshangul Guzum Regional State with the objectives of
isolation, identification and determination antimicrobial susceptibility profile of
Pasteurella multocida isolated from cattle infected with Hemorrhagic Septicemia.
The study was also aimed to detect the agent using classical PCR. Specimens were
collected purposively from cattle in areas with outbreak of Hemorrhagic septicemia
and a total of 384 swab samples were collected aseptically from cattle and transported
to Assosa Regional Veterinary Laboratory using a cold chain system. Specimens
were cultured on MacConkey agar and blood agar. Different biochemical tests were
also used in order to identify and characterize the agent of the suspected disease. The
prevalence of P.multocida was 13 (3.39%) out of 384 samples tested). All the isolates
were subjected to the in vitro antimicrobial ssensitivity tests and the result indicated
the presence of different degrees of susceptibility and resistance to most of the
antibacterial agents. The degree of susceptibility ranges from 15.4% for Tetracycline
upto 61.5% for Sterptomycin, and resistance is between 15.4% for Sterptomycin,
Clindamycin and Chloramphicol and 69.2% for Tetracycline. Similarly, (60 %) of
this discs showed the presence of multidrug resistance. The result of this test clearly
indicates that, Sterptomycin, Clindamycin and Chloramphicol could be prospective
drugs of choice. Analysis of PCR assay revealed the presence of P. multocida
serotype B2. In conclusion, the presence of P. multocida serotype B2 in the selected
areas can be considered as potential pathogens in causing Hemorrhagic septicemia in
cattle especially where there is no any practice of vaccination. Therefore, strict
measures like proper vaccination and antibiogram test to select effective drugs should
be implemented.
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Keywords
Antimicrobial sensitivity test, Benishangul Gumuz Regional State, Cattle Haemorrhagic Septicemia