Taxonomic and Demographic Studies on Three Species Complexes Within the Genus Alole L. (Aloaceae)in Ethiopia
| dc.contributor.advisor | Demissew Sebsebe (Professor) | |
| dc.contributor.advisor | Brysting Anne (Professor) | |
| dc.contributor.author | Dessalegn Fikre | |
| dc.date.accessioned | 2018-07-19T09:19:54Z | |
| dc.date.accessioned | 2023-11-18T09:50:16Z | |
| dc.date.available | 2018-07-19T09:19:54Z | |
| dc.date.available | 2023-11-18T09:50:16Z | |
| dc.date.issued | 2006-06 | |
| dc.description.abstract | Taxonomic and demographic studies were conducted on three species complexes within the genus Aloe L. (Aloaceae) in Ethiopia. The studies aimed to contribute new additional knowledge necessary to delineate the Aloe species and their conservation in the Flora of Ethiopia and Eritrea (FEE).In the taxonomic study, the status of 11 species was evaluated using morphological and molecular (AFLP) data. Morphological data were collected from herbarium specimens, fresh materials and literature. Data matrices were constructed for qualitative, quantitative and combined morphological data sets. Molecular (AFLP) data were obtained from leaves of randomly selected germinated seedlings. Total genomic DNA was extracted using a modified 2 x CTAB method described by Doyle and Doyle (1990). AFLP analysis was performed following a protocol modified from Vos et al. (1995). Fragments produced by each primer combination were analyzed and scored for the presence (1) or absence (0) of selected markers/fragments. Binary data matrices were constructed for each of the primer combinations and also for the combined data set. Each of the data matrices constructed for morphological and molecular (AFLP) data sets was subjected to multivariate analyses: cluster analysis (UPGMA) and principal coordinate analysis (PCO).The results from the analyses of both morphological and AFLP data supported the present status of nine out of the eleven species: A. harlana Reynolds, A. monticola Reynolds, A. debrana Christian, A. percrassa Tod., A. yavellana Reynolds, A. megalacantha Baker, A. gilbertii Sebsebe & Brandham, A. calidophila Reynolds and A. sinana Reynolds. The boundary between the two caulescent species from the northern part of the FEE area: A. camperi Schweinf. and A. adigratana Reynolds was not veryclear and particularly the genetic delimitation displayed close relationship. The neighborjoining tree constructed from combined AFLP data revealed thus weak support for A. camperi (51%) and A. adigratana (62%) but kept them together with high bootstrap support (94%). | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/9443 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Biology | en_US |
| dc.title | Taxonomic and Demographic Studies on Three Species Complexes Within the Genus Alole L. (Aloaceae)in Ethiopia | en_US |
| dc.type | Thesis | en_US |