The Effect of Crude Leaf Extracts of Osyris quadripartita Salzm. ex Decne against Plasmodium berghei inSwiss Albino Mice

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Addis Ababa Universty


Continuous emergence of multi-drug-resistant malaria parasites and their rapid spread across the globe warrant urgent search for new anti-malaria chemotherapeutics. Traditional medicinal plants have been the main sources for screening active phytochemicals against malaria. Accordingly, this study was aimed at evaluating the anti-malarial activity of Osyris quadripartita Decn.which is used for malaria treatment by local people around Fiche, north-central Ethiopia. Using aqueous, chloroform and methanol crude leaf extracts of the plant have been prepared and tested for toxicity and anti-malarial efficacy in Plasmodium berghei-infectedSwiss albino mice. Methanol solvent gave the highest yield of the plant extract. Acute toxicity study results indicated that the plant extract did not show any sign of toxicity up to 2000mg/kg. To assess the effect of the plants on the parasite, a 4-day suppressive standard test was performed. Data were analyzed using paired t-test and ANOVA. Both aqueous and methanol extract of O.quadripartita significantly (P<0.05) suppressed parasitemia and prevented packed cell volume (PCV) reduction and body weight gain in dose dependent manner.In addition, they prolonged the mean survival time. On the contrary, chloroform extract of O. quadripartita significantly (P<0.05) inhibited parasitemia, prevented body weight loss, prevented PCV reduction and prolonged survival time in all doses. The most efficacious extract was that of chloroform, which showed the highest parasitaemia suppression (41.26%) at 600mg/kg whereas its methanol extract caused 24.4% suppression at 200 mg/kg dose tested. Furthermore, aqueous extract of the plant showed 21.67% suppression at the same dose tested. The finding supports the traditional use of the plants for the treatment of malariathough parasite clearance was not achieved.Further evaluation of this plant is, however, needed before it is recommended for the control of malaria. Key words: Antimalarial activity, Osyris quadripartita,invivo, Plasmodium berghei, Swiss albino mice, Ethiopia



Antimalarial activity, Osyris quadripartita,invivo, Plasmodium berghei, Swiss albino mice, Ethiopia