Spectrophotometric Determination of Cholesterol with Dichromate
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Date
1988-07
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Publisher
Addis Ababa Universty
Abstract
The oxidation of cholesterol by dichromate has been
investigated for its application to quantitative determination
of cholesterol in blood sera. The redox system was
studied spectropho,tometrically by measuring the light apsorption
of the reduction product, chromium(III), at 585 nm
in benzene-acetic acid and pertoleum ether-acetic acid.
The effects of experiemntal variables have been studied
to establish the optimum conditions of the redox reaction.
It has been found that consistent and reproducible results
are obtained by carrying out the reaction at 800C for 1 hr.
It has also been found that petroleum ether can be used
as an alternative to benzene as a solvent to introduce
cholesterol into the reaction system. The coloured system
has been found to obey
tion range O.Ol-2.0mg ml-l of
Beer I S law in the con centracholesterol.
Other photometric
characteristics of the coloured system have also
been evaluated. On the basis of these studies a simple
and precise method has been developed for the determination
of cholesterol. The method ·has been applied to the
analysis of blood sera and the results obtained have been
compared with clinical results. The proposed method has
been found to give results which are in good agreement
with those of routinely used clinical methods.
Description
Keywords
Cholesterol with Dichromate