Molecular Detection of Peste Des Petitis Ruminants Virus in Outbreak Cases in the Pastoral Areas of Ethiopia
| dc.contributor.advisor | Dr. Kebede Amenu, Dr. Redeat Belaineh | |
| dc.contributor.author | Etsegenet, Tekeba | |
| dc.date.accessioned | 2020-11-12T06:48:58Z | |
| dc.date.accessioned | 2023-11-30T12:54:20Z | |
| dc.date.available | 2020-11-12T06:48:58Z | |
| dc.date.available | 2023-11-30T12:54:20Z | |
| dc.date.issued | 2020-06 | |
| dc.description.abstract | In this study a molecular detection of Peste des Petitis Ruminants virus was conducted from outbreak cases in the pastoral areas from January 2017 up to May 2019 with the objective of identifying the virus from outbreak cases from lowland areas using molecular techniques. A total of 60 swab samples were collected from nine outbreaks investigated in six regional states of the country. The outbreak investigation was made by the direct information received from regional veterinary laboratories after the confirmation of the outbreaks by pen-side test. From the total (n=21) samples tested using IcELISA, 18 (87%) were positive and from the total (n=60) samples tested for RT-PCR, 8 (13.3%) were found positive, showing great antigen detection rate difference between the two tests. It was also understood that the virus was detected in higher proportion in younger animals (66.6%) and the goat population (77%) than the rest. Additionally, the virus culture yielded a CPE result by growth on vero cells. The study was able to identify that PPR virus is circulating actively in the small ruminant population, special in the lowland areas, which has a great tendency to expand to the highland areas. Further characterization of the virus detected using molecular technique will help in better understanding of the disease and control program implementations for the future. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/23201 | |
| dc.language.iso | en | en_US |
| dc.subject | Lowland | en_US |
| dc.subject | Molecular | en_US |
| dc.subject | Outbreak | en_US |
| dc.subject | PPR | en_US |
| dc.subject | Small ruminant | en_US |
| dc.title | Molecular Detection of Peste Des Petitis Ruminants Virus in Outbreak Cases in the Pastoral Areas of Ethiopia | en_US |
| dc.type | Thesis | en_US |