Department of Biology

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Browsing Department of Biology by Subject "A376G/ G 202A"

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    Study of the Allelic Variants of Glucose-6-phosphate Dehydrogenase (G6PD) Using PCR-RFLP in the Oromia, Gambella and Benishangul Gumuz Regions of Ethiopia
    (Addis Ababa University, 2013) Tsegaye Wendimagegn; Belay Gurja (PhD); Assefa Ashenafi (PhD)
    Glucose-6-phosphate dehydrogenase (G6PD) deficiency has been considered as the most commoninherited enzymopathic disorder of the red blood cells, which affects more than 400 million people worldwide. Since the G6PD gene in human is X-linked gene more males are affected than females. G6PD deficiency is particularly prevalent in historically malaria-endemic areas. The main objective of this study is to study the allelic variants of Glucose -6- phosphate dehydrogenase (G6PD) locus prevalent in malaria endemic areas of Oromia, Gambella and Benishangul Gumuz regions in Ethiopia. In this study, blood sample of 620 individuals (326 females and 294 males) were collected from malaria endemic Oromia, Gambella and Benishangul Gumuz regions and stored at -20°C till used. Negative and positive samples were selected as the non-G6PD deficient and deficient control groups was tested for the African A type 376 G →A, African A- A376G & G 202A and 563C→T mutations. Molecular characterization of 620 individuals characterized to informative SNPs using PCR-RFLP techniques revealed an overall prevalence of G6PD Africa A (376 A→G) 10% with 0.0652 from Oromia, 10.5% with 0.074 from Gambella and 9.6% with 0.063 allele frequency from Benishangul Gumuz malaria endemic areas. The G6PD Africa A (376 A→G) was the most common, but African A- and Mediterranean type variants were not observed in this study. The G6PD variant identified in this study can contribute to the evidence-based use of antimalarial drugs like primaquine (for P. falciparum and P. vivax) treatment strategies that could greatly accelerate the elimination of malaria transmission in the study area. Population-based genotyping studies could be important to identify the distribution of dominant variants in the study regions. Keywords: Characterization, A376G/ G 202A, G6PD, PCR-RFLP, Prevalence Oromia, Ethiopia.