Browsing by Author "Tamirat,Mesfin"
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Item Analgesic and anti-inflammatory activities of Albizia gummifera (J. F. Gmel) C.A. Sm. bark hydro-alcoholic extract and its fractions in rodents(Addis Ababa University, 2023-10) Tamirat,Mesfin; Petros,Zelalem(PhD); Shibeshi,Workineh(PhD)Background: The majority of illnesses present as pain and inflammation. Despite advancement in pain medicines, there is a need for safe, effective analgesic drugs. In folkloric medicine, A.gummifera has been used for pain and inflammation, but not scientifically evaluated. Objectives: This study aimed to investigate the analgesic and anti-inflammatory activities of A.gummifera bark crude extract and its fractions in rodents. Methods: The analgesic activity of the A.gummifera was evaluated using an acetic-acid- induced writhing test and hot plate test by using acetyl salicylic acid 150 mg/kg and morphine 10 mg/kg as standard, respectively. Carrageenan-induced paw edema and cotton pellet granuloma methods were used to investigate the anti-inflammatory effect with Indomethacin 10 mg/kg and dexamethasone 0.5 mg/kg as the reference drugs, respectively. Three doses are selected based on the acute toxicity test result: 100 mg/kg, 200 mg/kg, and 400 mg/kg. Results: The A.gummifera bark crude extract and its fractions did not show any sign of toxicity at 2000 mg/kg. The crude extract and its fractions showed statistically significant analgesic and anti-inflammatory activities as compared to the control group (p<0.05). The crude extract and methanol fractions reduced writhes by 66.68% and elongate latency period by 61.47% on 60 and 90 minute, respectively. The maximum edema inhibition was 62.15% in the aqueous fraction after 2 hours of administration. Aqueous fraction produces maximum exudate and granuloma inhibition (62.07% and 65.66%, respectively). Conclusion: This study found that, A.gummifera bark crude extract and its fractions possessed promising analgesic and anti-inflammatory property.